Ethanol, growth hormone and testosterone in peripubertal rats

J. J. Tentler, N. LaPaglia, J. Steiner, D. Williams, M. Castelli, M. R. Kelley, N. V. Emanuele, M. A. Emanuele

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The deleterious effects of ethanol on the hypothalamic pituitary growth hormone axis in adult male humans and animals have been well documented. It is also well established that ethanol has toxic effects on testicular function in adult humans and animals. Much less is known, however, about the effects of ethanol on the growth hormone (GH) axis and testicular function in adolescence. Recent studies have established that adolescent problem drinking is a widespread and growing threat to the health of young people in the United States. In the present study, therefore, we investigated if acute ethanol exposure in peripubertal male Sprague-Dawley rats altered normal pituitary and testicular function. Serum levels of GH and testosterone were measured at 1.5, 3, 6, and 24 h after a single i.p. injection of either saline or 3 g/kg body weight ethanol. Histologic analysis as well as serum testosterone levels allowed us to assign animals to either early puberty (35-day-old animals), mid-puberty (41-day-old animals), or young adult (51- and 66-day-old animals) status. Ethanol produced significant decrements in serum testosterone in the 51- and 66-day-old animals, with a trend toward suppression in the 41-day-old group. Furthermore acute ethanol administration significantly decreased serum GH (P < 0.0001 by 3 way ANOVA) demonstrating a significant effect of ethanol on serum GH in all age groups and at all time points studied when compared with saline injected controls (P < 0.01 by Tukey's studentized range test). Despite this significant fall in peripheral GH levels, there was no decrease in either GH mRNA or growth hormone-releasing factor (GRF) mRNA levels nor in hypothalamic concentration of GRF peptide. We conclude that, as in adult animals, acute exposure to ethanol causes a prolonged and severe decrement in serum GH which is possibly mediated at the level of secretion. In addition, there is attenuation in testosterone secretion. These data are all the more important since GH and testosterone play critical roles in organ maturation during this stage of development.

Original languageEnglish (US)
Pages (from-to)477-487
Number of pages11
JournalJournal of Endocrinology
Volume152
Issue number3
DOIs
StatePublished - Mar 1997

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Growth Hormone
Testosterone
Ethanol
Serum
Growth Hormone-Releasing Hormone
Puberty
Hypothalamic Hormones
Messenger RNA
Poisons
Sprague Dawley Rats
Young Adult
Analysis of Variance
Age Groups
Body Weight
Injections
Health

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Tentler, J. J., LaPaglia, N., Steiner, J., Williams, D., Castelli, M., Kelley, M. R., ... Emanuele, M. A. (1997). Ethanol, growth hormone and testosterone in peripubertal rats. Journal of Endocrinology, 152(3), 477-487. https://doi.org/10.1677/joe.0.1520477

Ethanol, growth hormone and testosterone in peripubertal rats. / Tentler, J. J.; LaPaglia, N.; Steiner, J.; Williams, D.; Castelli, M.; Kelley, M. R.; Emanuele, N. V.; Emanuele, M. A.

In: Journal of Endocrinology, Vol. 152, No. 3, 03.1997, p. 477-487.

Research output: Contribution to journalArticle

Tentler, JJ, LaPaglia, N, Steiner, J, Williams, D, Castelli, M, Kelley, MR, Emanuele, NV & Emanuele, MA 1997, 'Ethanol, growth hormone and testosterone in peripubertal rats', Journal of Endocrinology, vol. 152, no. 3, pp. 477-487. https://doi.org/10.1677/joe.0.1520477
Tentler JJ, LaPaglia N, Steiner J, Williams D, Castelli M, Kelley MR et al. Ethanol, growth hormone and testosterone in peripubertal rats. Journal of Endocrinology. 1997 Mar;152(3):477-487. https://doi.org/10.1677/joe.0.1520477
Tentler, J. J. ; LaPaglia, N. ; Steiner, J. ; Williams, D. ; Castelli, M. ; Kelley, M. R. ; Emanuele, N. V. ; Emanuele, M. A. / Ethanol, growth hormone and testosterone in peripubertal rats. In: Journal of Endocrinology. 1997 ; Vol. 152, No. 3. pp. 477-487.
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