Ethanol impairs differentiation of human adipocyte stromal cells in culture

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Bioinformatic resources suggest that adipose tissue expresses mRNAs for alcohol dehydrogenases (ADHs) and ALDH2, and epidemiological studies indicate that heavy alcohol use reduces adipose tissue mass. We therefore characterized the expression of alcohol metabolizing enzymes in human, rat and mouse adipose tissue, preadipocytes, and adipocytes, the ability of adipocytes to metabolize ethanol, and the effects of ethanol on differentiation of human adipose stromal cells (hASCs). Methods: Adipose tissue, preadipocytes, and adipocytes were collected from rodents or from humans undergoing bariatric surgery. hASCs were differentiated in vitro using standard methods. Gene expression and cellular differentiation were analyzed by Western blotting, RT-PCR, and microscopy. Results: Class I ADH was expressed in human>mouse>rat adipose tissue, whereas ALDH2 was high in all samples. ADH, catalase, and ALDH2 were induced during differentiation of hASCs. The presence of 50mM ethanol markedly reduced the differentiation of hASCs; this effect was associated with inhibition of expression of transcription factors required for differentiation, but did not depend on the ability of the cells to metabolize ethanol. Conclusions: Human adipose tissue expresses alcohol oxidizing enzymes. The presence of ethanol at physiologically relevant concentrations inhibits differentiation of hASCs. Ethanol could alter adipose tissue biology, inducing a form of acquired lipodystrophy, which is consistent with epidemiological studies.

Original languageEnglish (US)
Pages (from-to)1584-1592
Number of pages9
JournalAlcoholism: Clinical and Experimental Research
Volume35
Issue number9
DOIs
StatePublished - Sep 2011
Externally publishedYes

Fingerprint

Stromal Cells
Adipocytes
Ethanol
Cell Culture Techniques
Tissue
Adipose Tissue
Alcohol Dehydrogenase
Alcohols
Rats
Epidemiologic Studies
Enzymes
Bioinformatics
Lipodystrophy
Gene expression
Catalase
Surgery
Bariatric Surgery
Microscopic examination
Transcription Factors
Computational Biology

Keywords

  • ADH
  • Adipose tissue
  • ALDH2
  • Catalase
  • Differentiation
  • Lipodystrophy

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Toxicology

Cite this

Ethanol impairs differentiation of human adipocyte stromal cells in culture. / Crabb, David; Zeng, Yan; Liangpunsakul, Suthat; Jones, RoseMarie; Considine, Robert.

In: Alcoholism: Clinical and Experimental Research, Vol. 35, No. 9, 09.2011, p. 1584-1592.

Research output: Contribution to journalArticle

@article{ebec0503a73941ce9a572d108b1db851,
title = "Ethanol impairs differentiation of human adipocyte stromal cells in culture",
abstract = "Background: Bioinformatic resources suggest that adipose tissue expresses mRNAs for alcohol dehydrogenases (ADHs) and ALDH2, and epidemiological studies indicate that heavy alcohol use reduces adipose tissue mass. We therefore characterized the expression of alcohol metabolizing enzymes in human, rat and mouse adipose tissue, preadipocytes, and adipocytes, the ability of adipocytes to metabolize ethanol, and the effects of ethanol on differentiation of human adipose stromal cells (hASCs). Methods: Adipose tissue, preadipocytes, and adipocytes were collected from rodents or from humans undergoing bariatric surgery. hASCs were differentiated in vitro using standard methods. Gene expression and cellular differentiation were analyzed by Western blotting, RT-PCR, and microscopy. Results: Class I ADH was expressed in human>mouse>rat adipose tissue, whereas ALDH2 was high in all samples. ADH, catalase, and ALDH2 were induced during differentiation of hASCs. The presence of 50mM ethanol markedly reduced the differentiation of hASCs; this effect was associated with inhibition of expression of transcription factors required for differentiation, but did not depend on the ability of the cells to metabolize ethanol. Conclusions: Human adipose tissue expresses alcohol oxidizing enzymes. The presence of ethanol at physiologically relevant concentrations inhibits differentiation of hASCs. Ethanol could alter adipose tissue biology, inducing a form of acquired lipodystrophy, which is consistent with epidemiological studies.",
keywords = "ADH, Adipose tissue, ALDH2, Catalase, Differentiation, Lipodystrophy",
author = "David Crabb and Yan Zeng and Suthat Liangpunsakul and RoseMarie Jones and Robert Considine",
year = "2011",
month = "9",
doi = "10.1111/j.1530-0277.2011.01504.x",
language = "English (US)",
volume = "35",
pages = "1584--1592",
journal = "Alcoholism: Clinical and Experimental Research",
issn = "0145-6008",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Ethanol impairs differentiation of human adipocyte stromal cells in culture

AU - Crabb, David

AU - Zeng, Yan

AU - Liangpunsakul, Suthat

AU - Jones, RoseMarie

AU - Considine, Robert

PY - 2011/9

Y1 - 2011/9

N2 - Background: Bioinformatic resources suggest that adipose tissue expresses mRNAs for alcohol dehydrogenases (ADHs) and ALDH2, and epidemiological studies indicate that heavy alcohol use reduces adipose tissue mass. We therefore characterized the expression of alcohol metabolizing enzymes in human, rat and mouse adipose tissue, preadipocytes, and adipocytes, the ability of adipocytes to metabolize ethanol, and the effects of ethanol on differentiation of human adipose stromal cells (hASCs). Methods: Adipose tissue, preadipocytes, and adipocytes were collected from rodents or from humans undergoing bariatric surgery. hASCs were differentiated in vitro using standard methods. Gene expression and cellular differentiation were analyzed by Western blotting, RT-PCR, and microscopy. Results: Class I ADH was expressed in human>mouse>rat adipose tissue, whereas ALDH2 was high in all samples. ADH, catalase, and ALDH2 were induced during differentiation of hASCs. The presence of 50mM ethanol markedly reduced the differentiation of hASCs; this effect was associated with inhibition of expression of transcription factors required for differentiation, but did not depend on the ability of the cells to metabolize ethanol. Conclusions: Human adipose tissue expresses alcohol oxidizing enzymes. The presence of ethanol at physiologically relevant concentrations inhibits differentiation of hASCs. Ethanol could alter adipose tissue biology, inducing a form of acquired lipodystrophy, which is consistent with epidemiological studies.

AB - Background: Bioinformatic resources suggest that adipose tissue expresses mRNAs for alcohol dehydrogenases (ADHs) and ALDH2, and epidemiological studies indicate that heavy alcohol use reduces adipose tissue mass. We therefore characterized the expression of alcohol metabolizing enzymes in human, rat and mouse adipose tissue, preadipocytes, and adipocytes, the ability of adipocytes to metabolize ethanol, and the effects of ethanol on differentiation of human adipose stromal cells (hASCs). Methods: Adipose tissue, preadipocytes, and adipocytes were collected from rodents or from humans undergoing bariatric surgery. hASCs were differentiated in vitro using standard methods. Gene expression and cellular differentiation were analyzed by Western blotting, RT-PCR, and microscopy. Results: Class I ADH was expressed in human>mouse>rat adipose tissue, whereas ALDH2 was high in all samples. ADH, catalase, and ALDH2 were induced during differentiation of hASCs. The presence of 50mM ethanol markedly reduced the differentiation of hASCs; this effect was associated with inhibition of expression of transcription factors required for differentiation, but did not depend on the ability of the cells to metabolize ethanol. Conclusions: Human adipose tissue expresses alcohol oxidizing enzymes. The presence of ethanol at physiologically relevant concentrations inhibits differentiation of hASCs. Ethanol could alter adipose tissue biology, inducing a form of acquired lipodystrophy, which is consistent with epidemiological studies.

KW - ADH

KW - Adipose tissue

KW - ALDH2

KW - Catalase

KW - Differentiation

KW - Lipodystrophy

UR - http://www.scopus.com/inward/record.url?scp=80052025718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052025718&partnerID=8YFLogxK

U2 - 10.1111/j.1530-0277.2011.01504.x

DO - 10.1111/j.1530-0277.2011.01504.x

M3 - Article

VL - 35

SP - 1584

EP - 1592

JO - Alcoholism: Clinical and Experimental Research

JF - Alcoholism: Clinical and Experimental Research

SN - 0145-6008

IS - 9

ER -