Ethanol Is self-administered into the nucleus accumbens shell, but not the core: Evidence of genetic sensitivity

Eric Engleman, Zheng Ming Ding, Scott M. Oster, Jamie E. Toalston, Richard Bell, James M. Murphy, William J. McBride, Zachary Rodd

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Background: A previous study indicated that selectively bred alcohol-preferring (P) rats self-administered ethanol (EtOH) directly into the posterior ventral tegmental area at lower concentrations than Wistar rats. The present study was undertaken to determine involvement of the nucleus accumbens (Acb) with EtOH reinforcement, and a relationship between genetic selection for high alcohol preference and sensitivity of the Acb to the reinforcing effects of EtOH. Methods: Adult P and Wistar rats were assigned to groups that self-infused 0 to 300 mg% EtOH into the Acb shell (AcbSh) or Acb Core (AcbC). Rats were placed into 2-lever (active and inactive) operant chambers and given EtOH for the first 4 sessions (acquisition), artificial cerebrospinal fluid (aCSF) for sessions 5 and 6 (extinction), and EtOH again in session 7 (reinstatement). Responding on the active lever produced a 100-nl injection of the infusate. Results: Alcohol-preferring rats self-infused 75 to 300 mg% EtOH, whereas Wistar rats reliably self-infused 100 and 300 mg% EtOH into the AcbSh. Both P and Wistar rats reduced responding on the active lever when aCSF was substituted for EtOH, and reinstated responding in session 7 when EtOH was restored. EtOH was not self-infused into the AcbC by P or Wistar rats. Conclusions: The present results indicate that the AcbSh, but not AcbC, is a neuroanatomical structure that mediates the reinforcing actions of EtOH. The data also suggest that, compared to Wistar rats, the AcbSh of P rats is more sensitive to the reinforcing effects of EtOH.

Original languageEnglish
Pages (from-to)2162-2171
Number of pages10
JournalAlcoholism: Clinical and Experimental Research
Volume33
Issue number12
DOIs
StatePublished - Dec 2009

Fingerprint

Nucleus Accumbens
Wistar Rats
Rats
Ethanol
Alcohols
Cerebrospinal Fluid
Cerebrospinal fluid
Ventral Tegmental Area
Genetic Selection
Injections
Reinforcement

Keywords

  • Alcohol-Preferring Rats
  • Ethanol
  • Intracranial Self-Administration
  • Nucleus Accumbens
  • Reinforcement

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Toxicology

Cite this

Ethanol Is self-administered into the nucleus accumbens shell, but not the core : Evidence of genetic sensitivity. / Engleman, Eric; Ding, Zheng Ming; Oster, Scott M.; Toalston, Jamie E.; Bell, Richard; Murphy, James M.; McBride, William J.; Rodd, Zachary.

In: Alcoholism: Clinical and Experimental Research, Vol. 33, No. 12, 12.2009, p. 2162-2171.

Research output: Contribution to journalArticle

Engleman, Eric ; Ding, Zheng Ming ; Oster, Scott M. ; Toalston, Jamie E. ; Bell, Richard ; Murphy, James M. ; McBride, William J. ; Rodd, Zachary. / Ethanol Is self-administered into the nucleus accumbens shell, but not the core : Evidence of genetic sensitivity. In: Alcoholism: Clinical and Experimental Research. 2009 ; Vol. 33, No. 12. pp. 2162-2171.
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abstract = "Background: A previous study indicated that selectively bred alcohol-preferring (P) rats self-administered ethanol (EtOH) directly into the posterior ventral tegmental area at lower concentrations than Wistar rats. The present study was undertaken to determine involvement of the nucleus accumbens (Acb) with EtOH reinforcement, and a relationship between genetic selection for high alcohol preference and sensitivity of the Acb to the reinforcing effects of EtOH. Methods: Adult P and Wistar rats were assigned to groups that self-infused 0 to 300 mg{\%} EtOH into the Acb shell (AcbSh) or Acb Core (AcbC). Rats were placed into 2-lever (active and inactive) operant chambers and given EtOH for the first 4 sessions (acquisition), artificial cerebrospinal fluid (aCSF) for sessions 5 and 6 (extinction), and EtOH again in session 7 (reinstatement). Responding on the active lever produced a 100-nl injection of the infusate. Results: Alcohol-preferring rats self-infused 75 to 300 mg{\%} EtOH, whereas Wistar rats reliably self-infused 100 and 300 mg{\%} EtOH into the AcbSh. Both P and Wistar rats reduced responding on the active lever when aCSF was substituted for EtOH, and reinstated responding in session 7 when EtOH was restored. EtOH was not self-infused into the AcbC by P or Wistar rats. Conclusions: The present results indicate that the AcbSh, but not AcbC, is a neuroanatomical structure that mediates the reinforcing actions of EtOH. The data also suggest that, compared to Wistar rats, the AcbSh of P rats is more sensitive to the reinforcing effects of EtOH.",
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T2 - Evidence of genetic sensitivity

AU - Engleman, Eric

AU - Ding, Zheng Ming

AU - Oster, Scott M.

AU - Toalston, Jamie E.

AU - Bell, Richard

AU - Murphy, James M.

AU - McBride, William J.

AU - Rodd, Zachary

PY - 2009/12

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N2 - Background: A previous study indicated that selectively bred alcohol-preferring (P) rats self-administered ethanol (EtOH) directly into the posterior ventral tegmental area at lower concentrations than Wistar rats. The present study was undertaken to determine involvement of the nucleus accumbens (Acb) with EtOH reinforcement, and a relationship between genetic selection for high alcohol preference and sensitivity of the Acb to the reinforcing effects of EtOH. Methods: Adult P and Wistar rats were assigned to groups that self-infused 0 to 300 mg% EtOH into the Acb shell (AcbSh) or Acb Core (AcbC). Rats were placed into 2-lever (active and inactive) operant chambers and given EtOH for the first 4 sessions (acquisition), artificial cerebrospinal fluid (aCSF) for sessions 5 and 6 (extinction), and EtOH again in session 7 (reinstatement). Responding on the active lever produced a 100-nl injection of the infusate. Results: Alcohol-preferring rats self-infused 75 to 300 mg% EtOH, whereas Wistar rats reliably self-infused 100 and 300 mg% EtOH into the AcbSh. Both P and Wistar rats reduced responding on the active lever when aCSF was substituted for EtOH, and reinstated responding in session 7 when EtOH was restored. EtOH was not self-infused into the AcbC by P or Wistar rats. Conclusions: The present results indicate that the AcbSh, but not AcbC, is a neuroanatomical structure that mediates the reinforcing actions of EtOH. The data also suggest that, compared to Wistar rats, the AcbSh of P rats is more sensitive to the reinforcing effects of EtOH.

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