Ethanol treatment of lymphoblastoid cell lines from alcoholics and non-alcoholics causes many subtle changes in gene expression

Jeanette McClintick, Andrew I. Brooks, Li Deng, Li Liang, Jen C. Wang, Manav Kapoor, Xiaoling Xuei, Tatiana Foroud, Jay A. Tischfield, Howard Edenberg

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To elucidate the effects of a controlled exposure to ethanol on gene expression, we studied lymphoblastoid cell lines (LCLs) from 21 alcoholics and 21 controls. We cultured each cell line for 24h with and without 75mM ethanol and measured gene expression using microarrays. Differences in expression between LCLs from alcoholics and controls included 13 genes previously identified as associated with alcoholism or related traits, including KCNA3, DICER1, ZNF415, CAT, SLC9A9, and PPARGC1B. The paired design allowed us to detect very small changes due to ethanol treatment: ethanol altered the expression of 37% of the probe sets (51% of the unique named genes) expressed in these LCLs, most by modest amounts. Ninety-nine percent of the named genes expressed in the LCLs were also expressed in brain. Key pathways affected by ethanol include cytokine, TNF, and NFκB signaling. Among the genes affected by ethanol were ANK3, EPHB1, SLC1A1, SLC9A9, NRD1, and SH3BP5, which were reported to be associated with alcoholism or related phenotypes in 2 genome-wide association studies. Genes that either differed in expression between alcoholics and controls or were affected by ethanol exposure are candidates for further study.

Original languageEnglish
Pages (from-to)603-610
Number of pages8
JournalAlcohol
Volume48
Issue number6
DOIs
StatePublished - Sep 1 2014

Fingerprint

Alcoholics
alcoholism
Gene expression
Ethanol
Cells
Genes
Gene Expression
Cell Line
cause
CAT
Therapeutics
Alcoholism
brain
Genome-Wide Association Study
candidacy
Microarrays
Cultured Cells
Brain
Cats
Association reactions

Keywords

  • Alcoholism
  • Cytokines
  • Gene expression
  • Lymphoblastoid cell lines
  • NFkappaB
  • TNF

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neurology
  • Toxicology
  • Health(social science)

Cite this

Ethanol treatment of lymphoblastoid cell lines from alcoholics and non-alcoholics causes many subtle changes in gene expression. / McClintick, Jeanette; Brooks, Andrew I.; Deng, Li; Liang, Li; Wang, Jen C.; Kapoor, Manav; Xuei, Xiaoling; Foroud, Tatiana; Tischfield, Jay A.; Edenberg, Howard.

In: Alcohol, Vol. 48, No. 6, 01.09.2014, p. 603-610.

Research output: Contribution to journalArticle

McClintick, Jeanette ; Brooks, Andrew I. ; Deng, Li ; Liang, Li ; Wang, Jen C. ; Kapoor, Manav ; Xuei, Xiaoling ; Foroud, Tatiana ; Tischfield, Jay A. ; Edenberg, Howard. / Ethanol treatment of lymphoblastoid cell lines from alcoholics and non-alcoholics causes many subtle changes in gene expression. In: Alcohol. 2014 ; Vol. 48, No. 6. pp. 603-610.
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