Ethnicity-dependent and -independent heterogeneity in healthy normal breast hierarchy impacts tumor characterization

Harikrishna Nakshatri, Manjushree Anjanappa, Poornima Bhat-Nakshatri

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recent reports of widespread genetic variation affecting regulation of gene expression raise the possibility of significant inter-individual differences in stem-progenitor-mature cell hierarchy in adult organs. This has not been explored because of paucity of methods to quantitatively assess subpopulation of normal epithelial cells on individual basis. We report the remarkable inter-individual differences in differentiation capabilities as documented by phenotypic heterogeneity in stem-progenitor-mature cell hierarchy of the normal breast. Ethnicity and genetic predisposition are partly responsible for this heterogeneity, evidenced by the finding that CD44+/CD24- and PROCR+/EpCAM- multi-potent stem cells were elevated significantly in African American women compared with Caucasians. ALDEFLUOR+ luminal stem/progenitor cells were lower in BRCA1-mutation carriers compared with cells from healthy donors (p = 0.0014). Moreover, tumor and adjoining-normal breast cells of the same patients showed distinct CD49f+/EpCAM+ progenitor, CD271+/EpCAM- basal, and ALDEFLUOR+ cell profiles. These inter-individual differences in the rate of differentiation in the normal breast may contribute to a substantial proportion of transcriptome, epigenome, and signaling pathway alterations and consequently has the potential to spuriously magnify the extent of documented tumor-specific gene expression. Therefore, comparative analysis of phenotypically defined subpopulations of normal and tumor cells on an individual basis may be required to identify cancer-specific aberrations.

Original languageEnglish (US)
Article number13526
JournalScientific reports
Volume5
DOIs
StatePublished - Aug 27 2015

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Breast
Stem Cells
Individuality
Neoplasms
Gene Expression Regulation
Genetic Predisposition to Disease
Transcriptome
African Americans
Epithelial Cells
Tissue Donors
Gene Expression
Mutation
Epithelial Cell Adhesion Molecule

ASJC Scopus subject areas

  • General

Cite this

Ethnicity-dependent and -independent heterogeneity in healthy normal breast hierarchy impacts tumor characterization. / Nakshatri, Harikrishna; Anjanappa, Manjushree; Bhat-Nakshatri, Poornima.

In: Scientific reports, Vol. 5, 13526, 27.08.2015.

Research output: Contribution to journalArticle

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