Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models

Keith M. Stantz, Bo Liu, Minsong Cao, Dan Reinecke, Mario Dzemidzic, Yun Liang, Robert Kruger

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Purpose: To evaluate photoacoustic CT spectroscopy (PCT-S) and dynamic contrast-enhanced CT (DCE-CT) ability to measure parameters - oxygen saturation and vascular physiology - associated with the intra-tumor oxygenation status. Material and Methods: Breast (VEGF165 enhance MCF-7) and ovarian (SKOV3x) cancer cells were implanted into the fat pads and flanks of immune deficient mice and allowed to grow to a diameter of 8-15 mm. CT was used to determine physiological parameters by acquiring a sequence of scans over a 10 minute period after an i.v. injection of a radio-opaque contrast agent (Isovue). These time-dependent contrast-enhanced curves were fit to a two-compartmental model determining tumor perfusion, fractional plasma volume, permeability-surface area produce, and fractional interstitial volume on a voxel-by-voxel basis. After which, the tumors were imaged using photoacoustic CT (Optosonics, Inc., Indianapolis, IN 46202). The near infrared spectra (700-910 nm) within the vasculature was fit to linear combination of measured oxy- and deoxy-hemoglobin blood samples to obtain oxygen saturation levels (SaO 2). Results: The PCT-S scanner was first calibrated using different samples of oxygenated blood, from which a statistical error ranging from 2.5-6.5% was measured and a plot of the hemoglobin dissociation curve was consistent with empirical formula. In vivo determination of tumor vasculature SaO 2 levels were measurably tracked, and spatially correlated to the periphery of the tumor. Tumor depend variations in SaO 2 - 0.32 (ovarian) and 0.60 (breast) - and in vascular physiology - perfusion, 1.03 and 0.063 mL/min/mL, and fractional plasma volume, 0.20 and 0.07 - were observed. Conclusion: Combined, PCT-S and CED-CT has the potential to measure intra-tumor levels of tumor oxygen saturation and vascular physiology, key parameters associated with hypoxia.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6143 I
DOIs
StatePublished - 2006
EventMedical Imaging 2006: Physiology, Function, and Structure from Medical Images - San Diego, CA, United States
Duration: Feb 12 2006Feb 14 2006

Other

OtherMedical Imaging 2006: Physiology, Function, and Structure from Medical Images
CountryUnited States
CitySan Diego, CA
Period2/12/062/14/06

Fingerprint

Photoacoustic effect
Tumors
Hemoglobin
Physiology
Spectroscopy
Oxygen
Blood
Plasmas
Oxygenation
Heterografts
Oils and fats
Cells
Infrared radiation

Keywords

  • Breast cancer
  • Dynamic contrast-enhanced CT
  • Hemoglobin
  • Ovarian cancer
  • Oxygen saturation
  • Photoacoustic spectroscopy

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Stantz, K. M., Liu, B., Cao, M., Reinecke, D., Dzemidzic, M., Liang, Y., & Kruger, R. (2006). Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6143 I). [61431F] https://doi.org/10.1117/12.654056

Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. / Stantz, Keith M.; Liu, Bo; Cao, Minsong; Reinecke, Dan; Dzemidzic, Mario; Liang, Yun; Kruger, Robert.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6143 I 2006. 61431F.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Stantz, KM, Liu, B, Cao, M, Reinecke, D, Dzemidzic, M, Liang, Y & Kruger, R 2006, Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6143 I, 61431F, Medical Imaging 2006: Physiology, Function, and Structure from Medical Images, San Diego, CA, United States, 2/12/06. https://doi.org/10.1117/12.654056
Stantz KM, Liu B, Cao M, Reinecke D, Dzemidzic M, Liang Y et al. Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6143 I. 2006. 61431F https://doi.org/10.1117/12.654056
Stantz, Keith M. ; Liu, Bo ; Cao, Minsong ; Reinecke, Dan ; Dzemidzic, Mario ; Liang, Yun ; Kruger, Robert. / Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6143 I 2006.
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abstract = "Purpose: To evaluate photoacoustic CT spectroscopy (PCT-S) and dynamic contrast-enhanced CT (DCE-CT) ability to measure parameters - oxygen saturation and vascular physiology - associated with the intra-tumor oxygenation status. Material and Methods: Breast (VEGF165 enhance MCF-7) and ovarian (SKOV3x) cancer cells were implanted into the fat pads and flanks of immune deficient mice and allowed to grow to a diameter of 8-15 mm. CT was used to determine physiological parameters by acquiring a sequence of scans over a 10 minute period after an i.v. injection of a radio-opaque contrast agent (Isovue). These time-dependent contrast-enhanced curves were fit to a two-compartmental model determining tumor perfusion, fractional plasma volume, permeability-surface area produce, and fractional interstitial volume on a voxel-by-voxel basis. After which, the tumors were imaged using photoacoustic CT (Optosonics, Inc., Indianapolis, IN 46202). The near infrared spectra (700-910 nm) within the vasculature was fit to linear combination of measured oxy- and deoxy-hemoglobin blood samples to obtain oxygen saturation levels (SaO 2). Results: The PCT-S scanner was first calibrated using different samples of oxygenated blood, from which a statistical error ranging from 2.5-6.5{\%} was measured and a plot of the hemoglobin dissociation curve was consistent with empirical formula. In vivo determination of tumor vasculature SaO 2 levels were measurably tracked, and spatially correlated to the periphery of the tumor. Tumor depend variations in SaO 2 - 0.32 (ovarian) and 0.60 (breast) - and in vascular physiology - perfusion, 1.03 and 0.063 mL/min/mL, and fractional plasma volume, 0.20 and 0.07 - were observed. Conclusion: Combined, PCT-S and CED-CT has the potential to measure intra-tumor levels of tumor oxygen saturation and vascular physiology, key parameters associated with hypoxia.",
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AU - Stantz, Keith M.

AU - Liu, Bo

AU - Cao, Minsong

AU - Reinecke, Dan

AU - Dzemidzic, Mario

AU - Liang, Yun

AU - Kruger, Robert

PY - 2006

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N2 - Purpose: To evaluate photoacoustic CT spectroscopy (PCT-S) and dynamic contrast-enhanced CT (DCE-CT) ability to measure parameters - oxygen saturation and vascular physiology - associated with the intra-tumor oxygenation status. Material and Methods: Breast (VEGF165 enhance MCF-7) and ovarian (SKOV3x) cancer cells were implanted into the fat pads and flanks of immune deficient mice and allowed to grow to a diameter of 8-15 mm. CT was used to determine physiological parameters by acquiring a sequence of scans over a 10 minute period after an i.v. injection of a radio-opaque contrast agent (Isovue). These time-dependent contrast-enhanced curves were fit to a two-compartmental model determining tumor perfusion, fractional plasma volume, permeability-surface area produce, and fractional interstitial volume on a voxel-by-voxel basis. After which, the tumors were imaged using photoacoustic CT (Optosonics, Inc., Indianapolis, IN 46202). The near infrared spectra (700-910 nm) within the vasculature was fit to linear combination of measured oxy- and deoxy-hemoglobin blood samples to obtain oxygen saturation levels (SaO 2). Results: The PCT-S scanner was first calibrated using different samples of oxygenated blood, from which a statistical error ranging from 2.5-6.5% was measured and a plot of the hemoglobin dissociation curve was consistent with empirical formula. In vivo determination of tumor vasculature SaO 2 levels were measurably tracked, and spatially correlated to the periphery of the tumor. Tumor depend variations in SaO 2 - 0.32 (ovarian) and 0.60 (breast) - and in vascular physiology - perfusion, 1.03 and 0.063 mL/min/mL, and fractional plasma volume, 0.20 and 0.07 - were observed. Conclusion: Combined, PCT-S and CED-CT has the potential to measure intra-tumor levels of tumor oxygen saturation and vascular physiology, key parameters associated with hypoxia.

AB - Purpose: To evaluate photoacoustic CT spectroscopy (PCT-S) and dynamic contrast-enhanced CT (DCE-CT) ability to measure parameters - oxygen saturation and vascular physiology - associated with the intra-tumor oxygenation status. Material and Methods: Breast (VEGF165 enhance MCF-7) and ovarian (SKOV3x) cancer cells were implanted into the fat pads and flanks of immune deficient mice and allowed to grow to a diameter of 8-15 mm. CT was used to determine physiological parameters by acquiring a sequence of scans over a 10 minute period after an i.v. injection of a radio-opaque contrast agent (Isovue). These time-dependent contrast-enhanced curves were fit to a two-compartmental model determining tumor perfusion, fractional plasma volume, permeability-surface area produce, and fractional interstitial volume on a voxel-by-voxel basis. After which, the tumors were imaged using photoacoustic CT (Optosonics, Inc., Indianapolis, IN 46202). The near infrared spectra (700-910 nm) within the vasculature was fit to linear combination of measured oxy- and deoxy-hemoglobin blood samples to obtain oxygen saturation levels (SaO 2). Results: The PCT-S scanner was first calibrated using different samples of oxygenated blood, from which a statistical error ranging from 2.5-6.5% was measured and a plot of the hemoglobin dissociation curve was consistent with empirical formula. In vivo determination of tumor vasculature SaO 2 levels were measurably tracked, and spatially correlated to the periphery of the tumor. Tumor depend variations in SaO 2 - 0.32 (ovarian) and 0.60 (breast) - and in vascular physiology - perfusion, 1.03 and 0.063 mL/min/mL, and fractional plasma volume, 0.20 and 0.07 - were observed. Conclusion: Combined, PCT-S and CED-CT has the potential to measure intra-tumor levels of tumor oxygen saturation and vascular physiology, key parameters associated with hypoxia.

KW - Breast cancer

KW - Dynamic contrast-enhanced CT

KW - Hemoglobin

KW - Ovarian cancer

KW - Oxygen saturation

KW - Photoacoustic spectroscopy

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