Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models

Keith M. Stantz, Bo Liu, Minsong Cao, Dan Reinecke, Mario Dzemidzic, Yun Liang, Robert Kruger

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Purpose: To evaluate photoacoustic CT spectroscopy (PCT-S) and dynamic contrast-enhanced CT (DCE-CT) ability to measure parameters - oxygen saturation and vascular physiology - associated with the intra-tumor oxygenation status. Material and Methods: Breast (VEGF165 enhance MCF-7) and ovarian (SKOV3x) cancer cells were implanted into the fat pads and flanks of immune deficient mice and allowed to grow to a diameter of 8-15 mm. CT was used to determine physiological parameters by acquiring a sequence of scans over a 10 minute period after an i.v. injection of a radio-opaque contrast agent (Isovue). These time-dependent contrast-enhanced curves were fit to a two-compartmental model determining tumor perfusion, fractional plasma volume, permeability-surface area produce, and fractional interstitial volume on a voxel-by-voxel basis. After which, the tumors were imaged using photoacoustic CT (Optosonics, Inc., Indianapolis, IN 46202). The near infrared spectra (700-910 nm) within the vasculature was fit to linear combination of measured oxy- and deoxy-hemoglobin blood samples to obtain oxygen saturation levels (SaO 2). Results: The PCT-S scanner was first calibrated using different samples of oxygenated blood, from which a statistical error ranging from 2.5-6.5% was measured and a plot of the hemoglobin dissociation curve was consistent with empirical formula. In vivo determination of tumor vasculature SaO 2 levels were measurably tracked, and spatially correlated to the periphery of the tumor. Tumor depend variations in SaO 2 - 0.32 (ovarian) and 0.60 (breast) - and in vascular physiology - perfusion, 1.03 and 0.063 mL/min/mL, and fractional plasma volume, 0.20 and 0.07 - were observed. Conclusion: Combined, PCT-S and CED-CT has the potential to measure intra-tumor levels of tumor oxygen saturation and vascular physiology, key parameters associated with hypoxia.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2006
Subtitle of host publicationPhysiology, Function, and Structure from Medical Images
DOIs
StatePublished - Jun 30 2006
EventMedical Imaging 2006: Physiology, Function, and Structure from Medical Images - San Diego, CA, United States
Duration: Feb 12 2006Feb 14 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6143 I
ISSN (Print)1605-7422

Other

OtherMedical Imaging 2006: Physiology, Function, and Structure from Medical Images
CountryUnited States
CitySan Diego, CA
Period2/12/062/14/06

Fingerprint

Photoacoustic effect
Tumors
Hemoglobin
Physiology
Spectroscopy
Oxygen
Blood
Plasmas
Oxygenation
Heterografts
Oils and fats
Cells
Infrared radiation

Keywords

  • Breast cancer
  • Dynamic contrast-enhanced CT
  • Hemoglobin
  • Ovarian cancer
  • Oxygen saturation
  • Photoacoustic spectroscopy

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Stantz, K. M., Liu, B., Cao, M., Reinecke, D., Dzemidzic, M., Liang, Y., & Kruger, R. (2006). Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. In Medical Imaging 2006: Physiology, Function, and Structure from Medical Images [61431F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6143 I). https://doi.org/10.1117/12.654056

Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. / Stantz, Keith M.; Liu, Bo; Cao, Minsong; Reinecke, Dan; Dzemidzic, Mario; Liang, Yun; Kruger, Robert.

Medical Imaging 2006: Physiology, Function, and Structure from Medical Images. 2006. 61431F (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6143 I).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Stantz, KM, Liu, B, Cao, M, Reinecke, D, Dzemidzic, M, Liang, Y & Kruger, R 2006, Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. in Medical Imaging 2006: Physiology, Function, and Structure from Medical Images., 61431F, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6143 I, Medical Imaging 2006: Physiology, Function, and Structure from Medical Images, San Diego, CA, United States, 2/12/06. https://doi.org/10.1117/12.654056
Stantz KM, Liu B, Cao M, Reinecke D, Dzemidzic M, Liang Y et al. Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. In Medical Imaging 2006: Physiology, Function, and Structure from Medical Images. 2006. 61431F. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.654056
Stantz, Keith M. ; Liu, Bo ; Cao, Minsong ; Reinecke, Dan ; Dzemidzic, Mario ; Liang, Yun ; Kruger, Robert. / Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models. Medical Imaging 2006: Physiology, Function, and Structure from Medical Images. 2006. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{1c84fbf0f0c94073947de48365f7f1d1,
title = "Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models",
abstract = "Purpose: To evaluate photoacoustic CT spectroscopy (PCT-S) and dynamic contrast-enhanced CT (DCE-CT) ability to measure parameters - oxygen saturation and vascular physiology - associated with the intra-tumor oxygenation status. Material and Methods: Breast (VEGF165 enhance MCF-7) and ovarian (SKOV3x) cancer cells were implanted into the fat pads and flanks of immune deficient mice and allowed to grow to a diameter of 8-15 mm. CT was used to determine physiological parameters by acquiring a sequence of scans over a 10 minute period after an i.v. injection of a radio-opaque contrast agent (Isovue). These time-dependent contrast-enhanced curves were fit to a two-compartmental model determining tumor perfusion, fractional plasma volume, permeability-surface area produce, and fractional interstitial volume on a voxel-by-voxel basis. After which, the tumors were imaged using photoacoustic CT (Optosonics, Inc., Indianapolis, IN 46202). The near infrared spectra (700-910 nm) within the vasculature was fit to linear combination of measured oxy- and deoxy-hemoglobin blood samples to obtain oxygen saturation levels (SaO 2). Results: The PCT-S scanner was first calibrated using different samples of oxygenated blood, from which a statistical error ranging from 2.5-6.5{\%} was measured and a plot of the hemoglobin dissociation curve was consistent with empirical formula. In vivo determination of tumor vasculature SaO 2 levels were measurably tracked, and spatially correlated to the periphery of the tumor. Tumor depend variations in SaO 2 - 0.32 (ovarian) and 0.60 (breast) - and in vascular physiology - perfusion, 1.03 and 0.063 mL/min/mL, and fractional plasma volume, 0.20 and 0.07 - were observed. Conclusion: Combined, PCT-S and CED-CT has the potential to measure intra-tumor levels of tumor oxygen saturation and vascular physiology, key parameters associated with hypoxia.",
keywords = "Breast cancer, Dynamic contrast-enhanced CT, Hemoglobin, Ovarian cancer, Oxygen saturation, Photoacoustic spectroscopy",
author = "Stantz, {Keith M.} and Bo Liu and Minsong Cao and Dan Reinecke and Mario Dzemidzic and Yun Liang and Robert Kruger",
year = "2006",
month = "6",
day = "30",
doi = "10.1117/12.654056",
language = "English (US)",
isbn = "0819461865",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Medical Imaging 2006",

}

TY - GEN

T1 - Evaluating dynamic contrast-enhanced and photoacoustic CT to assess intra-tumor heterogeneity in xenograft mouse models

AU - Stantz, Keith M.

AU - Liu, Bo

AU - Cao, Minsong

AU - Reinecke, Dan

AU - Dzemidzic, Mario

AU - Liang, Yun

AU - Kruger, Robert

PY - 2006/6/30

Y1 - 2006/6/30

N2 - Purpose: To evaluate photoacoustic CT spectroscopy (PCT-S) and dynamic contrast-enhanced CT (DCE-CT) ability to measure parameters - oxygen saturation and vascular physiology - associated with the intra-tumor oxygenation status. Material and Methods: Breast (VEGF165 enhance MCF-7) and ovarian (SKOV3x) cancer cells were implanted into the fat pads and flanks of immune deficient mice and allowed to grow to a diameter of 8-15 mm. CT was used to determine physiological parameters by acquiring a sequence of scans over a 10 minute period after an i.v. injection of a radio-opaque contrast agent (Isovue). These time-dependent contrast-enhanced curves were fit to a two-compartmental model determining tumor perfusion, fractional plasma volume, permeability-surface area produce, and fractional interstitial volume on a voxel-by-voxel basis. After which, the tumors were imaged using photoacoustic CT (Optosonics, Inc., Indianapolis, IN 46202). The near infrared spectra (700-910 nm) within the vasculature was fit to linear combination of measured oxy- and deoxy-hemoglobin blood samples to obtain oxygen saturation levels (SaO 2). Results: The PCT-S scanner was first calibrated using different samples of oxygenated blood, from which a statistical error ranging from 2.5-6.5% was measured and a plot of the hemoglobin dissociation curve was consistent with empirical formula. In vivo determination of tumor vasculature SaO 2 levels were measurably tracked, and spatially correlated to the periphery of the tumor. Tumor depend variations in SaO 2 - 0.32 (ovarian) and 0.60 (breast) - and in vascular physiology - perfusion, 1.03 and 0.063 mL/min/mL, and fractional plasma volume, 0.20 and 0.07 - were observed. Conclusion: Combined, PCT-S and CED-CT has the potential to measure intra-tumor levels of tumor oxygen saturation and vascular physiology, key parameters associated with hypoxia.

AB - Purpose: To evaluate photoacoustic CT spectroscopy (PCT-S) and dynamic contrast-enhanced CT (DCE-CT) ability to measure parameters - oxygen saturation and vascular physiology - associated with the intra-tumor oxygenation status. Material and Methods: Breast (VEGF165 enhance MCF-7) and ovarian (SKOV3x) cancer cells were implanted into the fat pads and flanks of immune deficient mice and allowed to grow to a diameter of 8-15 mm. CT was used to determine physiological parameters by acquiring a sequence of scans over a 10 minute period after an i.v. injection of a radio-opaque contrast agent (Isovue). These time-dependent contrast-enhanced curves were fit to a two-compartmental model determining tumor perfusion, fractional plasma volume, permeability-surface area produce, and fractional interstitial volume on a voxel-by-voxel basis. After which, the tumors were imaged using photoacoustic CT (Optosonics, Inc., Indianapolis, IN 46202). The near infrared spectra (700-910 nm) within the vasculature was fit to linear combination of measured oxy- and deoxy-hemoglobin blood samples to obtain oxygen saturation levels (SaO 2). Results: The PCT-S scanner was first calibrated using different samples of oxygenated blood, from which a statistical error ranging from 2.5-6.5% was measured and a plot of the hemoglobin dissociation curve was consistent with empirical formula. In vivo determination of tumor vasculature SaO 2 levels were measurably tracked, and spatially correlated to the periphery of the tumor. Tumor depend variations in SaO 2 - 0.32 (ovarian) and 0.60 (breast) - and in vascular physiology - perfusion, 1.03 and 0.063 mL/min/mL, and fractional plasma volume, 0.20 and 0.07 - were observed. Conclusion: Combined, PCT-S and CED-CT has the potential to measure intra-tumor levels of tumor oxygen saturation and vascular physiology, key parameters associated with hypoxia.

KW - Breast cancer

KW - Dynamic contrast-enhanced CT

KW - Hemoglobin

KW - Ovarian cancer

KW - Oxygen saturation

KW - Photoacoustic spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=33745376612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745376612&partnerID=8YFLogxK

U2 - 10.1117/12.654056

DO - 10.1117/12.654056

M3 - Conference contribution

AN - SCOPUS:33745376612

SN - 0819461865

SN - 9780819461865

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Medical Imaging 2006

ER -