Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori

Sonny K F Chong, Qinyuan Lou, Joseph F. Fitzgerald, Chao-Hung Lee

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The PCR primer set Hp1-Hp2, which amplifies a 109-bp fragment of the 16S rRNA gene of Helicobacter pylori, has been widely used for the detection of H. pylori in clinical specimens. We have examined 34 stool samples and 50 human tissue samples from H. pylori-infected and uninfected patients, five human leukocyte samples, and one human cell line by this PCR method. All of these specimens produced a 109-bp PCR product. When Escherichia coli DNA was used as the template, several nonspecific bands, but not the 109-bp band, were observed. No PCR products were generated when DNA samples from five different fungi were used as templates. These results indicate that this 109- bp PCR product was amplified from the human genome. The 109-bp PCR product generated from various clinical specimens also hybridized with the probe pHp, corresponding to a region internal to the PCR product of Hp1-Hp2. We conclude that the 16S rRNA gene PCR with the primer set Hp1-Hp2 is not specific and cannot be used to detect H. pylori in clinical specimens.

Original languageEnglish
Pages (from-to)2728-2730
Number of pages3
JournalJournal of Clinical Microbiology
Volume34
Issue number11
StatePublished - Nov 1996

Fingerprint

rRNA Genes
Helicobacter pylori
Polymerase Chain Reaction
DNA
Human Genome
Leukocytes
Fungi
Escherichia coli
Cell Line

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Chong, S. K. F., Lou, Q., Fitzgerald, J. F., & Lee, C-H. (1996). Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori. Journal of Clinical Microbiology, 34(11), 2728-2730.

Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori. / Chong, Sonny K F; Lou, Qinyuan; Fitzgerald, Joseph F.; Lee, Chao-Hung.

In: Journal of Clinical Microbiology, Vol. 34, No. 11, 11.1996, p. 2728-2730.

Research output: Contribution to journalArticle

Chong, SKF, Lou, Q, Fitzgerald, JF & Lee, C-H 1996, 'Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori', Journal of Clinical Microbiology, vol. 34, no. 11, pp. 2728-2730.
Chong, Sonny K F ; Lou, Qinyuan ; Fitzgerald, Joseph F. ; Lee, Chao-Hung. / Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 11. pp. 2728-2730.
@article{1238732b02dc466c92cf4eddf6fcddda,
title = "Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori",
abstract = "The PCR primer set Hp1-Hp2, which amplifies a 109-bp fragment of the 16S rRNA gene of Helicobacter pylori, has been widely used for the detection of H. pylori in clinical specimens. We have examined 34 stool samples and 50 human tissue samples from H. pylori-infected and uninfected patients, five human leukocyte samples, and one human cell line by this PCR method. All of these specimens produced a 109-bp PCR product. When Escherichia coli DNA was used as the template, several nonspecific bands, but not the 109-bp band, were observed. No PCR products were generated when DNA samples from five different fungi were used as templates. These results indicate that this 109- bp PCR product was amplified from the human genome. The 109-bp PCR product generated from various clinical specimens also hybridized with the probe pHp, corresponding to a region internal to the PCR product of Hp1-Hp2. We conclude that the 16S rRNA gene PCR with the primer set Hp1-Hp2 is not specific and cannot be used to detect H. pylori in clinical specimens.",
author = "Chong, {Sonny K F} and Qinyuan Lou and Fitzgerald, {Joseph F.} and Chao-Hung Lee",
year = "1996",
month = "11",
language = "English",
volume = "34",
pages = "2728--2730",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Evaluation of 16S rRNA gene PCR with primers Hp1 and Hp2 for detection of Helicobacter pylori

AU - Chong, Sonny K F

AU - Lou, Qinyuan

AU - Fitzgerald, Joseph F.

AU - Lee, Chao-Hung

PY - 1996/11

Y1 - 1996/11

N2 - The PCR primer set Hp1-Hp2, which amplifies a 109-bp fragment of the 16S rRNA gene of Helicobacter pylori, has been widely used for the detection of H. pylori in clinical specimens. We have examined 34 stool samples and 50 human tissue samples from H. pylori-infected and uninfected patients, five human leukocyte samples, and one human cell line by this PCR method. All of these specimens produced a 109-bp PCR product. When Escherichia coli DNA was used as the template, several nonspecific bands, but not the 109-bp band, were observed. No PCR products were generated when DNA samples from five different fungi were used as templates. These results indicate that this 109- bp PCR product was amplified from the human genome. The 109-bp PCR product generated from various clinical specimens also hybridized with the probe pHp, corresponding to a region internal to the PCR product of Hp1-Hp2. We conclude that the 16S rRNA gene PCR with the primer set Hp1-Hp2 is not specific and cannot be used to detect H. pylori in clinical specimens.

AB - The PCR primer set Hp1-Hp2, which amplifies a 109-bp fragment of the 16S rRNA gene of Helicobacter pylori, has been widely used for the detection of H. pylori in clinical specimens. We have examined 34 stool samples and 50 human tissue samples from H. pylori-infected and uninfected patients, five human leukocyte samples, and one human cell line by this PCR method. All of these specimens produced a 109-bp PCR product. When Escherichia coli DNA was used as the template, several nonspecific bands, but not the 109-bp band, were observed. No PCR products were generated when DNA samples from five different fungi were used as templates. These results indicate that this 109- bp PCR product was amplified from the human genome. The 109-bp PCR product generated from various clinical specimens also hybridized with the probe pHp, corresponding to a region internal to the PCR product of Hp1-Hp2. We conclude that the 16S rRNA gene PCR with the primer set Hp1-Hp2 is not specific and cannot be used to detect H. pylori in clinical specimens.

UR - http://www.scopus.com/inward/record.url?scp=0029956469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029956469&partnerID=8YFLogxK

M3 - Article

C2 - 8897173

AN - SCOPUS:0029956469

VL - 34

SP - 2728

EP - 2730

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 11

ER -