Evaluation of a commercial exoantigen test system for the rapid identification of systemic fungal pathogens

G. A. Denys, M. A. Newman, P. G. Standard

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Seventy-nine mycelial-form stock cultures of Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatum, and morphologically similar fungi were extracted and tested by using commercial macroimmunodiffusion exoantigen test kits and the Centers for Disease Control (CDC) reference system for identifying fungal isolates. Results showed 100% correlation between the two systems. Specific exoantigens of C. immitis and H. capsulatum extracted from agar slant cultures (slant extraction method) readily were identified. In eight of 26 cultures of B. dermatitidis, broth culture filtrates (broth-shake-flask method) were required to demonstrate the specific bands of identity. No false-negative reactions or cross-reactivity among the pathogens and other fungi were observed. The commercial test kits provided a rapid and specific method for identifying or confirming suspected fungal pathogens.

Original languageEnglish (US)
Pages (from-to)379-381
Number of pages3
JournalAmerican journal of clinical pathology
Volume79
Issue number3
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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