Evaluation of a real-time polymerase chain reaction assay using analyte-specific reagents for detection of methicillin-resistant Staphylococcus aureus nasal carriage

Tina Y. Fodrie, Stephen D. Allen, Deborah E. Blue, Patty Gary, Liang Cheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

OBJECTIVE: To validate a real-time polymerase chain reaction (PCR) assay for the detection of methicillinresistant Staphylococcus aureus (MRSA) that is capable of accommodating 30 patient samples in a single batch without compromising the level of sensitivity and specificity associated with PCR testing. STUDY DESIGN: A real-time PCR analyte-specific reagent (ASR) assay was compared to conventional culture methods using dual swab samples collected from the nares of 151 patients. RESULTS: Of the 151 specimens, 49 (32%) were positive for MRSA by PCR, whereas 40 (26%) were positive by culture methods. The Roche LightCycler 2.0 (Roche Diagnostics, Indianapolis, Indiana, U.S.A.) and its associated ASRs were capable of analyzing 30 patient samples and 2 controls in < 2 hours. Current data suggest that 4-10% of our patient specimens harbor both coagulase negative Staphylococcus and S aureus in the same sample. Samples such as this are reflexed to culture, only 24% of which contain MRSA. Efficiency in the laboratory was greatly improved. The sensitivity, specificity, positive predictive value and negative predictive value were 100%, 92%, 82% and 100%, respectively. CONCLUSION: The Roche LightCycler 2.0 PCR platform is a sensitive and efficient screening method for determining the nasal carriage of MRSA with a rapid turnaround time in the clinical laboratory.

Original languageEnglish
Pages (from-to)410-416
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Volume31
Issue number6
StatePublished - Dec 2009

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Nose
Staphylococcus aureus
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction
Sensitivity and Specificity
Coagulase
Reflex

Keywords

  • Methicillin-resistant Staphylococcus aureus
  • PCR
  • Roche LightCycler 2.0
  • Surveillance

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Evaluation of a real-time polymerase chain reaction assay using analyte-specific reagents for detection of methicillin-resistant Staphylococcus aureus nasal carriage. / Fodrie, Tina Y.; Allen, Stephen D.; Blue, Deborah E.; Gary, Patty; Cheng, Liang.

In: Analytical and Quantitative Cytology and Histology, Vol. 31, No. 6, 12.2009, p. 410-416.

Research output: Contribution to journalArticle

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