Evaluation of a reliable and cost-effective method of DNA isolation for mouse genotyping

Justin R. Sysol, Carrie Kempf, Michelle N. Helton, Yangbasai Dong, Daocheng Zhu, Haiying Sun, Joe G.N. Garcia, Roberto F. Machado, Jiwang Chen

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Genotyping is commonly used to define specific gene alterations or the presence of transgenes in mice. This procedure is typically done using DNA isolated from mouse tail tissue. Although there are commercially available kits for tail DNA isolation, they can be time consuming and costly for routine genotyping. In this study, we describe a rapid, "crude" DNA isolation method using mouse tail tissue and compare it to a frequently used, commercially available kit in the genotyping of over 1,000 total mice from 8 genetic lines. Our genotyping results were obtained faster and less expensively but with the same success rate (Crude method: 97. 7 %, Kit method: 98. 4 %). To our knowledge, this is the first systematic study to compare the reliability of this crude DNA isolation method for mouse genotyping compared to a commercially available kit.

Original languageEnglish (US)
Pages (from-to)509-514
Number of pages6
JournalBiotechnology Letters
Volume35
Issue number4
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Keywords

  • Genomic DNA isolation
  • Knockout mice
  • Mouse genotyping
  • PCR
  • Tail biopsy

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Sysol, J. R., Kempf, C., Helton, M. N., Dong, Y., Zhu, D., Sun, H., Garcia, J. G. N., Machado, R. F., & Chen, J. (2013). Evaluation of a reliable and cost-effective method of DNA isolation for mouse genotyping. Biotechnology Letters, 35(4), 509-514. https://doi.org/10.1007/s10529-012-1113-2