Evaluation of apoptosis of tumor and of apparently normal cells in human renal carcinoma

Stela Roman, Daniela Petrusca, Ioana Moldovan, Marius Paraoan, Amelia Petrescu, Daniel Damian, Niculae Noica, Andrei Sulica

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Apoptosis of tumor cells and of apparently normal renal cells (ANRC) isolated from the same kidney in 42 untreated patients with renal carcinomas (RC) was evaluated. Thirty five of the investigated tumors were of Grawitz type in different grades of differentiation. The intensity of the apoptotic process was routinely assessed by propidium iodide staining and flow- cytometry analysis. Similar results were obtained in the same cases by using TUNEL assay, by staining with annexin V and by DNA electrophoresis. In 85% of Grawitz carcinomas the proportion of apoptotic tumor cells was quite high, with mean% ± SD of 57.7 ± 27.3, whereas in transitional cell carcinoma of the bladder (TCC), the mean percentage of cells in apoptosis was of 22.3 ± 13.9. Unexpectedly, in ANRC displaying normal morphology and normal DNA content (diploidy), the mean% ± SD of apoptotic cells were found to exceed that of apoptotic tumor cells, 79.2 ± 21.6. The percentages of cells expressing Fas receptor and/or Fas ligand varied between large ranges in both tumor and ANRC, thus suggesting that other mechanisms are also involved in the activation of apoptosis. Immunohistochemical studies showed that the intensity of apoptosis correlated well with high p-53 and low bcl-2 expression. The intensity of apoptosis was generally not correlated with the cell proliferation index (S phase fraction), suggesting that in RC apoptosis can be activated in any stage of the cell cycle. Further investigations are necessary to understand the peculiar behaviour of tumor cells as well as of ANRC in renal carcinomas as compared to other types of malignancies.

Original languageEnglish (US)
Pages (from-to)15-22
Number of pages8
JournalImmunology Letters
Volume67
Issue number1
DOIs
StatePublished - Apr 1999
Externally publishedYes

Fingerprint

Apoptosis
Carcinoma
Kidney
Neoplasms
Renal Cell Carcinoma
Staining and Labeling
CD95 Antigens
Fas Ligand Protein
Propidium
Transitional Cell Carcinoma
Annexin A5
DNA
In Situ Nick-End Labeling
Diploidy
S Phase
Electrophoresis
Cell Cycle
Flow Cytometry
Urinary Bladder
Cell Proliferation

Keywords

  • Apoptosis
  • Apparently normal renal tissue
  • CD95
  • CD95L
  • Oncoproteins
  • Renal carcinoma

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Evaluation of apoptosis of tumor and of apparently normal cells in human renal carcinoma. / Roman, Stela; Petrusca, Daniela; Moldovan, Ioana; Paraoan, Marius; Petrescu, Amelia; Damian, Daniel; Noica, Niculae; Sulica, Andrei.

In: Immunology Letters, Vol. 67, No. 1, 04.1999, p. 15-22.

Research output: Contribution to journalArticle

Roman, S, Petrusca, D, Moldovan, I, Paraoan, M, Petrescu, A, Damian, D, Noica, N & Sulica, A 1999, 'Evaluation of apoptosis of tumor and of apparently normal cells in human renal carcinoma', Immunology Letters, vol. 67, no. 1, pp. 15-22. https://doi.org/10.1016/S0165-2478(98)00110-2
Roman, Stela ; Petrusca, Daniela ; Moldovan, Ioana ; Paraoan, Marius ; Petrescu, Amelia ; Damian, Daniel ; Noica, Niculae ; Sulica, Andrei. / Evaluation of apoptosis of tumor and of apparently normal cells in human renal carcinoma. In: Immunology Letters. 1999 ; Vol. 67, No. 1. pp. 15-22.
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AU - Damian, Daniel

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AU - Sulica, Andrei

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AB - Apoptosis of tumor cells and of apparently normal renal cells (ANRC) isolated from the same kidney in 42 untreated patients with renal carcinomas (RC) was evaluated. Thirty five of the investigated tumors were of Grawitz type in different grades of differentiation. The intensity of the apoptotic process was routinely assessed by propidium iodide staining and flow- cytometry analysis. Similar results were obtained in the same cases by using TUNEL assay, by staining with annexin V and by DNA electrophoresis. In 85% of Grawitz carcinomas the proportion of apoptotic tumor cells was quite high, with mean% ± SD of 57.7 ± 27.3, whereas in transitional cell carcinoma of the bladder (TCC), the mean percentage of cells in apoptosis was of 22.3 ± 13.9. Unexpectedly, in ANRC displaying normal morphology and normal DNA content (diploidy), the mean% ± SD of apoptotic cells were found to exceed that of apoptotic tumor cells, 79.2 ± 21.6. The percentages of cells expressing Fas receptor and/or Fas ligand varied between large ranges in both tumor and ANRC, thus suggesting that other mechanisms are also involved in the activation of apoptosis. Immunohistochemical studies showed that the intensity of apoptosis correlated well with high p-53 and low bcl-2 expression. The intensity of apoptosis was generally not correlated with the cell proliferation index (S phase fraction), suggesting that in RC apoptosis can be activated in any stage of the cell cycle. Further investigations are necessary to understand the peculiar behaviour of tumor cells as well as of ANRC in renal carcinomas as compared to other types of malignancies.

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