Evaluation of BSP expression and apoptosis in the periodontal ligament during orthodontic relapse

A preliminary study

A. Mcmanus, Achint Utreja, J. Chen, Z. Kalajzic, W. Yang, R. Nanda, S. Wadhwa, F. Uribe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Structured Abstract: Objective: To examine the expression of bone sialoprotein (BSP) and apoptosis in an in vivo orthodontic relapse model. Materials and Methods: Male mice (10-12 weeks old), either transgenic [green fluorescent protein (GFP) driven by the BSP promoter] or wild type, were used in this study. To achieve orthodontic tooth movement (OTM), maxillary right first molars were moved mesially using closed-coil springs. Animals were divided into an OTM group (14 days continuous orthodontic force - 11 animals) or Relapse group (10 days of force application followed by 4 days of relapse - 8 animals). The control group was comprised of the contralateral maxillary molars. The periodontal ligament (PDL) was analyzed in areas of compression and tension for transgenic expression, osteoclast localization, and the presence of apoptotic cells. Results: There was a significant decrease in GFP-labeled cells on the compression and tension sides of the PDL in the OTM group compared with control. In the relapse group, GFP-labeled cells were significantly decreased only on the old compression side. Osteoclasts were localized on the compression side of the OTM group, whereas in the Relapse group, they were present on both sides. PDL apoptosis significantly increased on the compression side in OTM and Relapse groups. Conclusion: Both OTM and Relapse groups exhibited a decreased number of GFP-labeled cells in areas of compression and tension. There was significant PDL apoptosis in regions under compressive forces following OTM and to a lesser extent following relapse.

Original languageEnglish (US)
Pages (from-to)239-248
Number of pages10
JournalOrthodontics and Craniofacial Research
Volume17
Issue number4
DOIs
StatePublished - Nov 1 2014
Externally publishedYes

Fingerprint

Integrin-Binding Sialoprotein
Periodontal Ligament
Tooth Movement Techniques
Orthodontics
Apoptosis
Recurrence
Green Fluorescent Proteins
Osteoclasts
Control Groups

Keywords

  • Apoptosis
  • Osteoblast
  • Osteoclast
  • Periodontal ligament
  • Tooth movement

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Otorhinolaryngology
  • Surgery

Cite this

Evaluation of BSP expression and apoptosis in the periodontal ligament during orthodontic relapse : A preliminary study. / Mcmanus, A.; Utreja, Achint; Chen, J.; Kalajzic, Z.; Yang, W.; Nanda, R.; Wadhwa, S.; Uribe, F.

In: Orthodontics and Craniofacial Research, Vol. 17, No. 4, 01.11.2014, p. 239-248.

Research output: Contribution to journalArticle

Mcmanus, A, Utreja, A, Chen, J, Kalajzic, Z, Yang, W, Nanda, R, Wadhwa, S & Uribe, F 2014, 'Evaluation of BSP expression and apoptosis in the periodontal ligament during orthodontic relapse: A preliminary study', Orthodontics and Craniofacial Research, vol. 17, no. 4, pp. 239-248. https://doi.org/10.1111/ocr.12049
Mcmanus, A. ; Utreja, Achint ; Chen, J. ; Kalajzic, Z. ; Yang, W. ; Nanda, R. ; Wadhwa, S. ; Uribe, F. / Evaluation of BSP expression and apoptosis in the periodontal ligament during orthodontic relapse : A preliminary study. In: Orthodontics and Craniofacial Research. 2014 ; Vol. 17, No. 4. pp. 239-248.
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AB - Structured Abstract: Objective: To examine the expression of bone sialoprotein (BSP) and apoptosis in an in vivo orthodontic relapse model. Materials and Methods: Male mice (10-12 weeks old), either transgenic [green fluorescent protein (GFP) driven by the BSP promoter] or wild type, were used in this study. To achieve orthodontic tooth movement (OTM), maxillary right first molars were moved mesially using closed-coil springs. Animals were divided into an OTM group (14 days continuous orthodontic force - 11 animals) or Relapse group (10 days of force application followed by 4 days of relapse - 8 animals). The control group was comprised of the contralateral maxillary molars. The periodontal ligament (PDL) was analyzed in areas of compression and tension for transgenic expression, osteoclast localization, and the presence of apoptotic cells. Results: There was a significant decrease in GFP-labeled cells on the compression and tension sides of the PDL in the OTM group compared with control. In the relapse group, GFP-labeled cells were significantly decreased only on the old compression side. Osteoclasts were localized on the compression side of the OTM group, whereas in the Relapse group, they were present on both sides. PDL apoptosis significantly increased on the compression side in OTM and Relapse groups. Conclusion: Both OTM and Relapse groups exhibited a decreased number of GFP-labeled cells in areas of compression and tension. There was significant PDL apoptosis in regions under compressive forces following OTM and to a lesser extent following relapse.

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