Evaluation of Helicobacter hepaticus bacterial shedding in fostered and sex-segregated C57BL/6 mice

Robin Crisler-Roberts, Zhongming Ge, Michael T. Kearney, Kem B. Singletary, James G. Fox, Christopher S. Roberts, David G. Baker

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Neonatal fostering has been evaluated as a means of eliminating Helicobacter hepaticus infection in laboratory mouse colonies. The purpose of the present study was to evaluate cross-fostering of neonatal C57BL/6 pups from experimentally infected dams after male-absent parturition and to determine the effects of sex and housing strategy on H. hepaticus populations. Approximately 20 C57BL/6 mice (age, 1 to 4 days) were fostered daily. In all fostered mice, fecal samples collected at 21 and 42 days of age and cecal samples collected at 42 days of age tested negative for H. hepaticus by polymerase chain reaction analysis. Our results demonstrate that removal of the male prior to parturition extends the fostering period to yield Helicobacter-free mice. In a second experiment, the effects of time of infection, housing strategy, and sex on fecal H. hepaticus shedding and cecal colonization were evaluated. Neither time nor housing strategy affected bacterial shedding. In contrast, fecal and cecal bacterial loads were higher in male mice versus female mice. A novel predictive algorithm was developed to predict cecal bacterial colonization levels in light of fecal bacterial loads. Our findings likely will prove useful in Helicobacter eradication efforts and in studies designed to further elucidate the role of H. hepaticus in disease.

Original languageEnglish
Pages (from-to)515-522
Number of pages8
JournalComparative Medicine
Volume55
Issue number6
StatePublished - Dec 2005

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Bacterial Shedding
Helicobacter hepaticus
bacterial shedding
Inbred C57BL Mouse
Foster Home Care
gender
mice
Helicobacter
Bacterial Load
Polymerase chain reaction
Dams
Parturition
parturition
Helicobacter Infections
bacterial colonization
pups
infection
Polymerase Chain Reaction
Experiments
polymerase chain reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Crisler-Roberts, R., Ge, Z., Kearney, M. T., Singletary, K. B., Fox, J. G., Roberts, C. S., & Baker, D. G. (2005). Evaluation of Helicobacter hepaticus bacterial shedding in fostered and sex-segregated C57BL/6 mice. Comparative Medicine, 55(6), 515-522.

Evaluation of Helicobacter hepaticus bacterial shedding in fostered and sex-segregated C57BL/6 mice. / Crisler-Roberts, Robin; Ge, Zhongming; Kearney, Michael T.; Singletary, Kem B.; Fox, James G.; Roberts, Christopher S.; Baker, David G.

In: Comparative Medicine, Vol. 55, No. 6, 12.2005, p. 515-522.

Research output: Contribution to journalArticle

Crisler-Roberts, R, Ge, Z, Kearney, MT, Singletary, KB, Fox, JG, Roberts, CS & Baker, DG 2005, 'Evaluation of Helicobacter hepaticus bacterial shedding in fostered and sex-segregated C57BL/6 mice', Comparative Medicine, vol. 55, no. 6, pp. 515-522.
Crisler-Roberts, Robin ; Ge, Zhongming ; Kearney, Michael T. ; Singletary, Kem B. ; Fox, James G. ; Roberts, Christopher S. ; Baker, David G. / Evaluation of Helicobacter hepaticus bacterial shedding in fostered and sex-segregated C57BL/6 mice. In: Comparative Medicine. 2005 ; Vol. 55, No. 6. pp. 515-522.
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