Evaluation of Her2 status using photoacoustic spectroscopic CT techniques

Michael Shaffer, Robert Kruger, Daniel Reinecke, Helen Chin-Sinex, Marc Mendonca, Keith M. Stantz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Purpose: The purpose of this study is to determine the feasibility of using photacoustic CT spectroscopy(PCT-s) to track a near infrared dye conjugated with trastuzumab in vivo. Materials and Methods: An animal model was developed which contained both high and low Her2 expression tumor xenografts on the same mouse. The tumors were imaged at multiple wavelengths (680- 950nm) in the PCT scanner one day prior to injection of the near infrared conjugated probe. Baseline optical imaging data was acquired and the probe was then injected via the tail vein. Fluorescence data was acquired over the next week, PCT spectroscopic data was also acquired during this timeframe. The mice were sacrificed and tumors were extirpated and sent to pathology for IHC staining to verify Her2 expression levels. The optical fluorescence images were analyzed to determine probe uptake dynamics. Reconstructed PCT spectroscopic data was analyzed using IDL routines to deconvolve the probe signal from endogenous background signals, and to determine oxygen saturation. Results: The location of the NIR conjugate was able to be identified within the tumor utilizing IDL fitting routines, in addition oxygen saturation, and hemoglobin concentrations were discernible from the spectroscopic data. Conclusion: Photacoustic spectroscopy allows for the determination of in vivo tumor drug delivery at greater depths than can be determined from optical imaging techniques.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7564
DOIs
StatePublished - 2010
EventPhotons Plus Ultrasound: Imaging and Sensing 2010 - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2010
CountryUnited States
CitySan Francisco, CA
Period1/24/101/26/10

Fingerprint

Photoacoustic effect
Tumors
tumors
evaluation
probes
Optical Imaging
Neoplasms
mice
Spectrum Analysis
Fluorescence
Spectroscopy
Oxygen
Infrared radiation
saturation
Imaging techniques
fluorescence
animal models
Hemoglobin
hemoglobin
pathology

Keywords

  • Her2
  • IRDye 800
  • Photoacoustic imaging

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Shaffer, M., Kruger, R., Reinecke, D., Chin-Sinex, H., Mendonca, M., & Stantz, K. M. (2010). Evaluation of Her2 status using photoacoustic spectroscopic CT techniques. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7564). [756435] https://doi.org/10.1117/12.842639

Evaluation of Her2 status using photoacoustic spectroscopic CT techniques. / Shaffer, Michael; Kruger, Robert; Reinecke, Daniel; Chin-Sinex, Helen; Mendonca, Marc; Stantz, Keith M.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7564 2010. 756435.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shaffer, M, Kruger, R, Reinecke, D, Chin-Sinex, H, Mendonca, M & Stantz, KM 2010, Evaluation of Her2 status using photoacoustic spectroscopic CT techniques. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7564, 756435, Photons Plus Ultrasound: Imaging and Sensing 2010, San Francisco, CA, United States, 1/24/10. https://doi.org/10.1117/12.842639
Shaffer M, Kruger R, Reinecke D, Chin-Sinex H, Mendonca M, Stantz KM. Evaluation of Her2 status using photoacoustic spectroscopic CT techniques. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7564. 2010. 756435 https://doi.org/10.1117/12.842639
Shaffer, Michael ; Kruger, Robert ; Reinecke, Daniel ; Chin-Sinex, Helen ; Mendonca, Marc ; Stantz, Keith M. / Evaluation of Her2 status using photoacoustic spectroscopic CT techniques. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7564 2010.
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