Evaluation of microglia/macrophage cells from rat striatum and prefrontal cortex reveals differential expression of inflammatory-related mrna after methamphetamine

Joanne S. Kays, Bryan K. Yamamoto

Research output: Contribution to journalArticlepeer-review

Abstract

RNA sequencing (RNAseq) can be a powerful tool in the identification of transcriptional changes after drug treatment. RNAseq was utilized to determine expression changes in Fluorescence-activated cell sorted (FACS) CD11b/c+ cells from the striatum (STR) and prefrontal cortex (PFC) of male Sprague-Dawley rats after a methamphetamine (METH) binge dosing regimen. Resident microglia and infiltrating macrophages were collected 2 h or 3 days after drug administration. Gene expression changes indicated there was an increase toward an overall proinflammatory state, or M1 polarization, along with what appears to be a subset of cells that differentiated toward the anti-inflammatory M2 polarization. In general, there were significantly more mRNA expression changes in the STR than the PFC and more at 2 h post-binge METH than at 3 days post-binge METH. Additionally, Ingenuity® Pathway Analysis along with details of RNA expression changes revealed cyclo-oxygenase 2 (COX2)-driven prostaglandin (PG) E2 synthesis, glutamine uptake, and the Nuclear factor erythroid2-related factor 2 (NRF2) canonical pathway in microglia were associated with the binge administration regimen of METH.

Original languageEnglish (US)
Article number340
JournalBrain Sciences
Volume9
Issue number12
DOIs
StatePublished - Dec 2019

Keywords

  • Inflammation
  • Macrophage
  • Methamphetamine
  • Microglia
  • RNA sequencing

ASJC Scopus subject areas

  • Neuroscience(all)

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