Evaluation of nitrone spin-trapping agents as radioprotectors

H. K. Young, R. A. Floyd, M. L. Maidt, Joseph Dynlacht

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The focus of this investigation was to determine whether the nitrone spin-trapping compounds α-phenyl-N-tert-butylnitrone (PBN), 5,5-dimethyl-1- pyrroline-N-oxide (DMPO) and α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN) are radioprotectors. Two methods were used to assess for radioprotection: measurement of oxidative damage to DNA bases and mammalian cell survival assays. Oxidative damage to DNA was quantified by measuring the relative amounts of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) produced by the reaction of hydroxyl radicals (OH) with 2-deoxyguanosine (dG) after irradiation. PBN, DMPO and POBN, when dissolved in aqueous solutions of either dG or naked salmon sperm DNA, reduced the formation of 8-OH-dG by 137Cs γ irradiation significantly. The spin-trapping agents, especially PBN at lower concentrations, were more effective in preventing radiation- induced formation of 8-OH-dG in naked DNA than in free dG. These data suggest that PBN, DMPO and POBN act as free radical scavengers which may associate with DNA and afford protection against γ rays. However, no enhancement of survival was observed when Chinese hamster ovary (CHO) cells were exposed to high non-toxic concentrations of PBN or POBN prior to and during irradiation with 60Co γ rays and scored for clonogenic survival. DMPO provided only minimal protection from radiation-induced cell killing.

Original languageEnglish (US)
Pages (from-to)227-231
Number of pages5
JournalRadiation Research
Volume146
Issue number2
StatePublished - Aug 1996
Externally publishedYes

Fingerprint

Spin Trapping
Deoxyguanosine
Oxides
oxides
trapping
evaluation
deoxyribonucleic acid
DNA
irradiation
DNA Damage
rays
Free Radical Scavengers
Radiation Protection
damage
Salmon
ovaries
Cricetulus
nitrones
spin trapping
hamsters

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Young, H. K., Floyd, R. A., Maidt, M. L., & Dynlacht, J. (1996). Evaluation of nitrone spin-trapping agents as radioprotectors. Radiation Research, 146(2), 227-231.

Evaluation of nitrone spin-trapping agents as radioprotectors. / Young, H. K.; Floyd, R. A.; Maidt, M. L.; Dynlacht, Joseph.

In: Radiation Research, Vol. 146, No. 2, 08.1996, p. 227-231.

Research output: Contribution to journalArticle

Young, HK, Floyd, RA, Maidt, ML & Dynlacht, J 1996, 'Evaluation of nitrone spin-trapping agents as radioprotectors', Radiation Research, vol. 146, no. 2, pp. 227-231.
Young, H. K. ; Floyd, R. A. ; Maidt, M. L. ; Dynlacht, Joseph. / Evaluation of nitrone spin-trapping agents as radioprotectors. In: Radiation Research. 1996 ; Vol. 146, No. 2. pp. 227-231.
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