Evaluation of plasma lysophospholipids for diagnostic significance using electrospray ionization mass spectrometry (ESI-MS) analyses

Yijin Xiao, Yonghong Chen, Alexander W. Kennedy, Jerome Belinson, Yan Xu

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

We previously reported that lysophosphatidic acid (LPA) represents a potential biomarker for ovarian and other gynecologic cancers. To further improve the accuracy and potentially increase the sensitivity and specificity of the assay, we developed an electrospray ionization mass spectrometry (ESI-MS)-based method to analyze LPA and related lysophospholipids. LPA, lysophosphatidylinositol (LPI), lysophosphatidylserine (LPS), and lysophosphatidylcholine (LPC) could be detected with high sensitivity (in low pmol range) using this method. Standard curves were established for quantitative analysis. LPA and closely related lysophospholipids isolated from thin-layer chromatography (TLC) plates were analyzed directly by ESI-MS. This ESI-MS-based assay allows simultaneous detection and quantitation of all different species of LPAs and LPIs in a sample over a range of at least 5-300 pmol. Moreover, this test was at least 50 times more sensitive when a multiple reaction monitoring (MRM) mode was used. Using these protocols in a limited set of analyses, we found that both LPA and LPI were elevated in patients with ovarian cancer.

Original languageEnglish (US)
Pages (from-to)242-259
Number of pages18
JournalAnnals of the New York Academy of Sciences
Volume905
StatePublished - 2000
Externally publishedYes

Fingerprint

Lysophospholipids
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Mass spectrometry
Plasmas
Assays
Thin layer chromatography
Lysophosphatidylcholines
Biomarkers
Thin Layer Chromatography
Ovarian Neoplasms
lysophosphatidic acid
Evaluation
Mass Spectrometry
Diagnostics
Plasma
Sensitivity and Specificity
Monitoring
Chemical analysis
Cancer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Evaluation of plasma lysophospholipids for diagnostic significance using electrospray ionization mass spectrometry (ESI-MS) analyses. / Xiao, Yijin; Chen, Yonghong; Kennedy, Alexander W.; Belinson, Jerome; Xu, Yan.

In: Annals of the New York Academy of Sciences, Vol. 905, 2000, p. 242-259.

Research output: Contribution to journalArticle

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