Evaluation of T2Candida Panel for detection of Candida in peritoneal dialysates

Anne M. Kouri, Theodore W. Kieffer, Corina Nailescu, Jeffrey Leiser, Bryan H. Schmitt, Ryan F. Relich, Thomas E. Davis, John J. Manaloor

Research output: Contribution to journalArticle

Abstract

Fungal peritonitis in the peritoneal dialysis population is difficult to diagnose promptly due to the inherently slow cultivation-based methods currently required for identification of peritonitis pathogens. Because of the moderate risk for severe complications, the need for rapid diagnostics is considerable. One possible solution to this unmet need is the T2Candida Panel, a new technology designed to detect the most common pathogenic Candida spp. directly from whole blood specimens in as little as a few hours. We hypothesized that this technology could be applied to the detection of Candida in peritoneal dialysate, a matrix not currently approved by the Food and Drug Administration for testing by this system. Remnant dialysate samples from three healthy (noninfected) pediatric peritoneal dialysis patients were spiked with Candida glabrata, serially diluted, and tested in triplicate with unaltered dialysate specimens. The assay detected C. glabrata in 100% of spiked dialysate samples across the full spectrum of dilutions tested, and no assay inhibition or cross-reactivity was noted. These findings suggest one of possibly more applications of this technology. The positive clinical implications of this test will continue to be realized as its use is validated in peritoneal dialysate and other patient specimen types.

Original languageEnglish (US)
Pages (from-to)96-99
Number of pages4
JournalPeritoneal Dialysis International
Volume40
Issue number1
DOIs
StatePublished - Jan 1 2020

Keywords

  • Candida peritonitis
  • fungal peritonitis
  • peritonitis
  • T2Candida
  • T2Dx

ASJC Scopus subject areas

  • Nephrology

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