Evaluation of the Digene Hybrid Capture II assay with the Rapid Capture System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae

B. Van Der Pol, J. A. Williams, N. J. Smith, Byron Batteiger, A. P. Cullen, H. Erdman, T. Edens, K. Davis, H. Salim-Hammad, V. W. Chou, L. Scearce, J. Blutman, W. J. Payne

Research output: Contribution to journalArticle

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Abstract

Screening for chlamydial and gonococcal infection has been strongly recommended for all sexually active women under the age of 26. Advances in the ability to detect infection by nucleic acid detection techniques have improved access to screening methods in routine clinical practices. To meet the increasing demand for testing, a high-throughput system is desirable. We evaluated the performance of the Hybrid Capture 2 CT/GC (HC2) assay with the Digene Rapid Capture System (HC2-RCS). The results of HC2-RCS for endocervical samples from 330 women were compared to those of culture and the COBAS Amplicor PCR. For detection of chlamydial infection, HC2-RCS had a sensitivity and a specificity similar to those of PCR (P > 0.5) and an improved sensitivity compared to that of culture (P = 0.007). For identification of gonococcal infections, all assays performed similarly (P > 0.5). The performance of HC2-RCS was also compared to that of the manual HC2 format (HC2-M) with these samples and with 911 endocervical samples collected previously. The performance of HC2-RCS was equivalent to that of HC2-M; the overall concordance rates for the detection of chlamydia and gonorrhea were 99.7% (κ = 0.97) and 99.8% (κ = 0.97), respectively. When the HC2 assay was performed with a semiautomated system application designed for high throughput, it demonstrated high sensitivity and a high specificity for detection of both Chlamydia trachomatis and Neisseria gonorrhoeae.

Original languageEnglish
Pages (from-to)3558-3564
Number of pages7
JournalJournal of Clinical Microbiology
Volume40
Issue number10
DOIs
StatePublished - Oct 1 2002

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Neisseria gonorrhoeae
Chlamydia trachomatis
Infection
Polymerase Chain Reaction
Chlamydia
Gonorrhea
Nucleic Acids
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Evaluation of the Digene Hybrid Capture II assay with the Rapid Capture System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. / Van Der Pol, B.; Williams, J. A.; Smith, N. J.; Batteiger, Byron; Cullen, A. P.; Erdman, H.; Edens, T.; Davis, K.; Salim-Hammad, H.; Chou, V. W.; Scearce, L.; Blutman, J.; Payne, W. J.

In: Journal of Clinical Microbiology, Vol. 40, No. 10, 01.10.2002, p. 3558-3564.

Research output: Contribution to journalArticle

Van Der Pol, B, Williams, JA, Smith, NJ, Batteiger, B, Cullen, AP, Erdman, H, Edens, T, Davis, K, Salim-Hammad, H, Chou, VW, Scearce, L, Blutman, J & Payne, WJ 2002, 'Evaluation of the Digene Hybrid Capture II assay with the Rapid Capture System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae', Journal of Clinical Microbiology, vol. 40, no. 10, pp. 3558-3564. https://doi.org/10.1128/JCM.40.10.3558-3564.2002
Van Der Pol, B. ; Williams, J. A. ; Smith, N. J. ; Batteiger, Byron ; Cullen, A. P. ; Erdman, H. ; Edens, T. ; Davis, K. ; Salim-Hammad, H. ; Chou, V. W. ; Scearce, L. ; Blutman, J. ; Payne, W. J. / Evaluation of the Digene Hybrid Capture II assay with the Rapid Capture System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 10. pp. 3558-3564.
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AU - Batteiger, Byron

AU - Cullen, A. P.

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AU - Edens, T.

AU - Davis, K.

AU - Salim-Hammad, H.

AU - Chou, V. W.

AU - Scearce, L.

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