Evaluation of the mirn23a cluster through an iTRAQ-based quantitative proteomic approach

Katelyn R. Ludwig, Richard Dahl, Amanda B. Hummon

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that are implicated in a number of disease states. MiRNAs can exist as individual entities or may be clustered and transcribed as a single polycistron. The mirn23a cluster consists of three miRNAs: miR-23a, miR-24-2, and miR-27a. Although these miRNAs are transcribed together, they often exist at varying levels in the cell. Despite the fact that the mirn23a cluster is known to play a role in a number of diseases and developmental processes, few direct targets have been identified. In this study, we examined the effects of miR-23a, miR-24-2, miR-27a, or the mirn23a cluster overexpression on the proteome of 70Z/3 pre-B lymphoblast cells. Quantitative mass spectrometry using isobaric tags for relative and absolute quantification (iTRAQ) allowed for the global profiling of cell lines after miRNA overexpression. We identified a number of targets of each miRNA that contained predicted miRNA seed sequences and are likely direct targets. In addition, we discovered a cohort of shared miRNA targets and cluster targets, demonstrating the importance of studying miRNA clusters in their entirety.

Original languageEnglish (US)
Pages (from-to)1497-1505
Number of pages9
JournalJournal of Proteome Research
Volume15
Issue number5
DOIs
StatePublished - May 6 2016

Keywords

  • cluster
  • iTRAQ
  • miR-23a
  • miR-24
  • miR-27a
  • microRNA

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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