Evaluation of the neutrophil-lymphocyte ratio as a measure of distress in rats

Melissa P. Swan, Debra Hickman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The ability to evaluate distress in laboratory animals is needed in order to ensure that husbandry and experimental procedures do not negatively impact animal welfare. Accurate measurement of acute stress and chronic stress, and distinguishing between stress that is harmful (distress) and stress that does no harm (eustress), can be challenging. Whereas corticosterone concentrations are commonly used to measure stress in laboratory animals, the neutrophil-lymphocyte ratio has been proposed as a potentially better indicator of chronic stress. Furthermore, an association between such measures of stress and concurrent behavioral indicators of negative welfare is required to determine their accuracy in evaluating distress. The authors compared serum corticosterone concentrations and neutrophil-lymphocyte ratios to assess acute or chronic stress in male Sprague Dawley rats. Elevated serum corticosterone concentrations, but not neutrophil-lymphocyte ratios, were associated with acute stress exposure, whereas elevated neutrophil-lymphocyte ratios, but not serum corticosterone concentrations, were associated with chronic stress exposure. Because the neutrophil-lymphocyte ratio differences corresponded with a behavioral indicator of distress in chronically stressed rats, it may serve as a valuable tool for the physiological assessment of distress in rats.

Original languageEnglish
Pages (from-to)276-282
Number of pages7
JournalLab Animal
Volume43
Issue number8
DOIs
StatePublished - 2014

Fingerprint

distress
neutrophils
corticosterone
Corticosterone
Neutrophils
lymphocytes
Lymphocytes
rats
Laboratory Animals
laboratory animals
Serum
Animal Welfare
animal welfare
Sprague Dawley Rats

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Medicine(all)

Cite this

Evaluation of the neutrophil-lymphocyte ratio as a measure of distress in rats. / Swan, Melissa P.; Hickman, Debra.

In: Lab Animal, Vol. 43, No. 8, 2014, p. 276-282.

Research output: Contribution to journalArticle

@article{0bfbe7eac38b4e41a7284868d8c5f946,
title = "Evaluation of the neutrophil-lymphocyte ratio as a measure of distress in rats",
abstract = "The ability to evaluate distress in laboratory animals is needed in order to ensure that husbandry and experimental procedures do not negatively impact animal welfare. Accurate measurement of acute stress and chronic stress, and distinguishing between stress that is harmful (distress) and stress that does no harm (eustress), can be challenging. Whereas corticosterone concentrations are commonly used to measure stress in laboratory animals, the neutrophil-lymphocyte ratio has been proposed as a potentially better indicator of chronic stress. Furthermore, an association between such measures of stress and concurrent behavioral indicators of negative welfare is required to determine their accuracy in evaluating distress. The authors compared serum corticosterone concentrations and neutrophil-lymphocyte ratios to assess acute or chronic stress in male Sprague Dawley rats. Elevated serum corticosterone concentrations, but not neutrophil-lymphocyte ratios, were associated with acute stress exposure, whereas elevated neutrophil-lymphocyte ratios, but not serum corticosterone concentrations, were associated with chronic stress exposure. Because the neutrophil-lymphocyte ratio differences corresponded with a behavioral indicator of distress in chronically stressed rats, it may serve as a valuable tool for the physiological assessment of distress in rats.",
author = "Swan, {Melissa P.} and Debra Hickman",
year = "2014",
doi = "10.1038/laban.529",
language = "English",
volume = "43",
pages = "276--282",
journal = "Lab Animal",
issn = "0093-7355",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - Evaluation of the neutrophil-lymphocyte ratio as a measure of distress in rats

AU - Swan, Melissa P.

AU - Hickman, Debra

PY - 2014

Y1 - 2014

N2 - The ability to evaluate distress in laboratory animals is needed in order to ensure that husbandry and experimental procedures do not negatively impact animal welfare. Accurate measurement of acute stress and chronic stress, and distinguishing between stress that is harmful (distress) and stress that does no harm (eustress), can be challenging. Whereas corticosterone concentrations are commonly used to measure stress in laboratory animals, the neutrophil-lymphocyte ratio has been proposed as a potentially better indicator of chronic stress. Furthermore, an association between such measures of stress and concurrent behavioral indicators of negative welfare is required to determine their accuracy in evaluating distress. The authors compared serum corticosterone concentrations and neutrophil-lymphocyte ratios to assess acute or chronic stress in male Sprague Dawley rats. Elevated serum corticosterone concentrations, but not neutrophil-lymphocyte ratios, were associated with acute stress exposure, whereas elevated neutrophil-lymphocyte ratios, but not serum corticosterone concentrations, were associated with chronic stress exposure. Because the neutrophil-lymphocyte ratio differences corresponded with a behavioral indicator of distress in chronically stressed rats, it may serve as a valuable tool for the physiological assessment of distress in rats.

AB - The ability to evaluate distress in laboratory animals is needed in order to ensure that husbandry and experimental procedures do not negatively impact animal welfare. Accurate measurement of acute stress and chronic stress, and distinguishing between stress that is harmful (distress) and stress that does no harm (eustress), can be challenging. Whereas corticosterone concentrations are commonly used to measure stress in laboratory animals, the neutrophil-lymphocyte ratio has been proposed as a potentially better indicator of chronic stress. Furthermore, an association between such measures of stress and concurrent behavioral indicators of negative welfare is required to determine their accuracy in evaluating distress. The authors compared serum corticosterone concentrations and neutrophil-lymphocyte ratios to assess acute or chronic stress in male Sprague Dawley rats. Elevated serum corticosterone concentrations, but not neutrophil-lymphocyte ratios, were associated with acute stress exposure, whereas elevated neutrophil-lymphocyte ratios, but not serum corticosterone concentrations, were associated with chronic stress exposure. Because the neutrophil-lymphocyte ratio differences corresponded with a behavioral indicator of distress in chronically stressed rats, it may serve as a valuable tool for the physiological assessment of distress in rats.

UR - http://www.scopus.com/inward/record.url?scp=84904906476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904906476&partnerID=8YFLogxK

U2 - 10.1038/laban.529

DO - 10.1038/laban.529

M3 - Article

C2 - 25050728

AN - SCOPUS:84904906476

VL - 43

SP - 276

EP - 282

JO - Lab Animal

JF - Lab Animal

SN - 0093-7355

IS - 8

ER -