Evaluation of the repertoire of the TonB-dependent receptors of Haemophilus ducreyi for their role in virulence in humans

Isabelle Leduc, Keith E. Banks, Kate R. Fortney, Kristine B. Patterson, Steve D. Billings, Barry P. Katz, Stanley M. Spinola, Christopher Elkins

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Haemophilus ducreyi contains 3 TonB-dependent receptors: the hemoglobin receptor HgbA, which is required for virulence in humans; the heme receptor TdhA; and an uncharacterized conserved hypothetical protein TdX (HD0646). A double tdX/tdhA mutant (FX527) was constructed on the background of a human-passaged variant of strain 35000 (35000HP). Six volunteers were infected with 35000HP at 3 sites on one arm and with FX527 at 3 sites on the other. The pustule formation rate was 55.6% (95% confidence interval [CI], 35.7%-75.4%) at 18 parent-strain sites and 44.4% (95% CI, 15.0%-73.9%) at 18 mutant-strain sites (P = .51). Similar amounts of 35000HP and FX527 were recovered from pustules in semiquantitative culture. Thus, TdX and TdhA are not necessary for virulence, whereas HgbA is both necessary and sufficient for virulence in humans. The data suggest that hemoglobin is the sole source of heme/iron used by H. ducreyi in vivo and has implications for the potential of HgbA as a vaccine.

Original languageEnglish (US)
Pages (from-to)1103-1109
Number of pages7
JournalJournal of Infectious Diseases
Volume197
Issue number8
DOIs
StatePublished - Apr 15 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Leduc, I., Banks, K. E., Fortney, K. R., Patterson, K. B., Billings, S. D., Katz, B. P., Spinola, S. M., & Elkins, C. (2008). Evaluation of the repertoire of the TonB-dependent receptors of Haemophilus ducreyi for their role in virulence in humans. Journal of Infectious Diseases, 197(8), 1103-1109. https://doi.org/10.1086/586901