Evaluation of the repertoire of the TonB-dependent receptors of Haemophilus ducreyi for their role in virulence in humans

Isabelle Leduc, Keith E. Banks, Kate R. Fortney, Kristine B. Patterson, Steve D. Billings, Barry Katz, Stanley Spinola, Christopher Elkins

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Haemophilus ducreyi contains 3 TonB-dependent receptors: the hemoglobin receptor HgbA, which is required for virulence in humans; the heme receptor TdhA; and an uncharacterized conserved hypothetical protein TdX (HD0646). A double tdX/tdhA mutant (FX527) was constructed on the background of a human-passaged variant of strain 35000 (35000HP). Six volunteers were infected with 35000HP at 3 sites on one arm and with FX527 at 3 sites on the other. The pustule formation rate was 55.6% (95% confidence interval [CI], 35.7%-75.4%) at 18 parent-strain sites and 44.4% (95% CI, 15.0%-73.9%) at 18 mutant-strain sites (P = .51). Similar amounts of 35000HP and FX527 were recovered from pustules in semiquantitative culture. Thus, TdX and TdhA are not necessary for virulence, whereas HgbA is both necessary and sufficient for virulence in humans. The data suggest that hemoglobin is the sole source of heme/iron used by H. ducreyi in vivo and has implications for the potential of HgbA as a vaccine.

Original languageEnglish
Pages (from-to)1103-1109
Number of pages7
JournalJournal of Infectious Diseases
Volume197
Issue number8
DOIs
StatePublished - Apr 15 2008

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Haemophilus ducreyi
Virulence
Hemoglobins
Confidence Intervals
Heme
Volunteers
Vaccines
Iron
Proteins

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Evaluation of the repertoire of the TonB-dependent receptors of Haemophilus ducreyi for their role in virulence in humans. / Leduc, Isabelle; Banks, Keith E.; Fortney, Kate R.; Patterson, Kristine B.; Billings, Steve D.; Katz, Barry; Spinola, Stanley; Elkins, Christopher.

In: Journal of Infectious Diseases, Vol. 197, No. 8, 15.04.2008, p. 1103-1109.

Research output: Contribution to journalArticle

Leduc, Isabelle ; Banks, Keith E. ; Fortney, Kate R. ; Patterson, Kristine B. ; Billings, Steve D. ; Katz, Barry ; Spinola, Stanley ; Elkins, Christopher. / Evaluation of the repertoire of the TonB-dependent receptors of Haemophilus ducreyi for their role in virulence in humans. In: Journal of Infectious Diseases. 2008 ; Vol. 197, No. 8. pp. 1103-1109.
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