Evaluation of urothelial stretch-induced cyclooxygenase-2 expression in a mouse ureteral obstruction model

Samuel P. Sterrett, Eric R. Wilkinson, Travis Jerde, William S. Mellon, Stephen Y. Nakada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Prostanoids play a major role in the nociceptive response to ureteral obstruction. Cyclooxygenases (COXs) 1 and 2 catalyze the rate-limiting step in prostanoid synthesis; COX-2 is the more inducible isoform. Previous studies in human and animal models have shown that COX-2 is highly induced during ureteral obstruction. Our objective was to characterize acute COX-2 induction in a reproducible mouse model. Unilateral ureteral ligation was performed, and obstruction was maintained for 2, 4, 6, 8, or 12 hours. We evaluated COX-2 protein expression using Western immunoblotting, and found that ureteral obstruction induced COX-2 expression ninefold within 6 hours. This is the first report to characterize in vivo temporal stretch-induced COX-2 expression in a mouse model. This model will be critical for elucidation of COX-2 signaling pathways and may eventually help to identify novel therapeutic targets for treating ureteral obstruction.

Original languageEnglish (US)
Pages (from-to)541-544
Number of pages4
JournalJournal of Endourology
Volume23
Issue number3
DOIs
StatePublished - Mar 1 2009
Externally publishedYes

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Ureteral Obstruction
Cyclooxygenase 2
Prostaglandins
Cyclooxygenase 1
Ligation
Protein Isoforms
Animal Models
Western Blotting

ASJC Scopus subject areas

  • Urology
  • Medicine(all)

Cite this

Evaluation of urothelial stretch-induced cyclooxygenase-2 expression in a mouse ureteral obstruction model. / Sterrett, Samuel P.; Wilkinson, Eric R.; Jerde, Travis; Mellon, William S.; Nakada, Stephen Y.

In: Journal of Endourology, Vol. 23, No. 3, 01.03.2009, p. 541-544.

Research output: Contribution to journalArticle

Sterrett, Samuel P. ; Wilkinson, Eric R. ; Jerde, Travis ; Mellon, William S. ; Nakada, Stephen Y. / Evaluation of urothelial stretch-induced cyclooxygenase-2 expression in a mouse ureteral obstruction model. In: Journal of Endourology. 2009 ; Vol. 23, No. 3. pp. 541-544.
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