Evidence for an autocrine/paracrine role for interleukin-6 in bone resorption by giant cells from giant cell tumors of bone

Y. Ohsaki, S. Takahashi, T. Scarcez, A. Demulder, T. Nishihara, R. Williams, G. David Roodman

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Interleukin-6 (IL-6) is a multifunctional cytokine whose role in osteoclastic bone resorption has not been clearly defined. Therefore, we have used giant cells, which express many features of osteoclasts, from giant cell tumors of bone as a model to examine the role that IL-6 may play in human osteoclastic bone resorption. We found that conditioned medium from 24-h cultures of highly purified giant cells (106/ml) contained large amounts of IL-6 (37.9 ± 8.8 ng/ml), similar to the amount of IL-6 produced by tumor stromal cells (29.8 ± 11.5 ng/ ml). Giant cells and stromal cells from giant cell tumors expressed IL-6 mRNA, as indicated by polymerase chain reaction analysis and in situ hybridization studies, and immunohistochemical techniques demonstrated that the giant cells expressed IL-6 receptors. The addition of a neutralizing antibody to IL-6 significantly decreased the area of dentine resorbed by purified giant cells in a dose-dependent manner, and the addition of IL-6 to cultures of purified giant cells pretreated with anti-IL-6 restored the resorbing capacity of the giant cells. These data suggest that IL-6 may act as both an autocrine and a paracrine factor for human osteoclasts and play an important role in the boneresorbing capacity of these cells.

Original languageEnglish (US)
Pages (from-to)2229-2234
Number of pages6
JournalEndocrinology
Volume131
Issue number5
StatePublished - Nov 1992
Externally publishedYes

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Giant Cell Tumor of Bone
Giant Cells
Bone Resorption
Interleukin-6
Osteoclasts
Stromal Cells
Interleukin-6 Receptors
Giant Cell Tumors
Dentin
Conditioned Culture Medium
Neutralizing Antibodies
In Situ Hybridization
Cytokines

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ohsaki, Y., Takahashi, S., Scarcez, T., Demulder, A., Nishihara, T., Williams, R., & Roodman, G. D. (1992). Evidence for an autocrine/paracrine role for interleukin-6 in bone resorption by giant cells from giant cell tumors of bone. Endocrinology, 131(5), 2229-2234.

Evidence for an autocrine/paracrine role for interleukin-6 in bone resorption by giant cells from giant cell tumors of bone. / Ohsaki, Y.; Takahashi, S.; Scarcez, T.; Demulder, A.; Nishihara, T.; Williams, R.; Roodman, G. David.

In: Endocrinology, Vol. 131, No. 5, 11.1992, p. 2229-2234.

Research output: Contribution to journalArticle

Ohsaki, Y, Takahashi, S, Scarcez, T, Demulder, A, Nishihara, T, Williams, R & Roodman, GD 1992, 'Evidence for an autocrine/paracrine role for interleukin-6 in bone resorption by giant cells from giant cell tumors of bone', Endocrinology, vol. 131, no. 5, pp. 2229-2234.
Ohsaki Y, Takahashi S, Scarcez T, Demulder A, Nishihara T, Williams R et al. Evidence for an autocrine/paracrine role for interleukin-6 in bone resorption by giant cells from giant cell tumors of bone. Endocrinology. 1992 Nov;131(5):2229-2234.
Ohsaki, Y. ; Takahashi, S. ; Scarcez, T. ; Demulder, A. ; Nishihara, T. ; Williams, R. ; Roodman, G. David. / Evidence for an autocrine/paracrine role for interleukin-6 in bone resorption by giant cells from giant cell tumors of bone. In: Endocrinology. 1992 ; Vol. 131, No. 5. pp. 2229-2234.
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