Evidence for both a regulatory mutation and a structural mutation in a family with maple syrup urine disease

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Maple syrup urine disease (MSUD) results from a deficiency of branched chain α-ketoacid dehydrogenase (BCKDH). We have studied the etiology of MSUD by determining the enzyme activity, protein, and mRNA levels of BCKDH in fibroblasts from a classic MSUD patient and his parents. By enzymatic amplification of the patient's mRNA followed by cloning and DNA sequencing, we have identified a T to A transversion that alters a tyrosine to an asparagine at residue 394 of the E1α subunit. Amplification of both mRNA and genomic DNA, in combination with allele-specific oligonucleotide hybridization, demonstrated that the father was heterozygous for this mutant allele. The mother was homozygous for the allele encoding the normal Tyr394, but expressed only about half of the normal level of mRNA and protein. The patient was genetically heterozygous for this altered allele, although only the abnormal allele was expressed as mRNA. We conclude that the patient was a compound heterozygote, inheriting an allele encoding an abnormal E1α from the father, and an allele from the mother containing a cis-acting defect in regulation which abolished the expression of one of the E1α alleles. Our results revealed for the first time that a case of MSUD was caused by structural and regulatory mutations involving the E1α subunit.

Original languageEnglish (US)
Pages (from-to)1425-1429
Number of pages5
JournalJournal of Clinical Investigation
Issue number4
StatePublished - 1989

ASJC Scopus subject areas

  • Medicine(all)

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