Evidence for involvement of the epidermal platelet Activating factor receptor in ultraviolet-B-radiation induced interleukin-8 production

Nicholas B. Countryman, Yong Pei, Qiaofang Yi, Dan Spandau, Jeffrey Travers

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Ultraviolet B radiation has been shown to generate cutaneous inflammation in part through inducing oxidative stress and cytokine production in human keratinocytes. Amongst the proinflammatory cytokines synthesized in response to ultraviolet B radiation is the potent chemoattractant interleukin-8. Though the lipid mediator platelet-activating factor (PAF) is synthesized in response to oxidative stress, and keratinocytes express PAF receptors linked to cytokine biosynthesis, it is not known whether PAF is involved in ultraviolet-B-induced epidermal cell cytokine production. These studies examined the role of the PAF system in ultraviolet-B-induced epidermal cell interleukin-8 biosynthesis using a novel model system created by retroviral-mediated transduction of the PAF-receptor-negative human epidermal cell line KB with the human PAF receptor. Treatment of PAF-receptor-expressing KB cells with the metabolically stable PAF receptor agonist carbamoyl-PAF resulted in increased interleukin-8 mRNA and protein, indicating that activation of the epidermal PAF receptor was linked to interleukin-8 production. Ultraviolet B irradiation of PAF-receptor-expressing KB cells resulted in significant increases in both interleukin-8 mRNA and protein in comparison to ultraviolet-B-treated control KB cells. Pretreatment with PAF receptor antagonists inhibited both carbamoyl-PAF-induced and ultraviolet-B-induced interleukin-8 production in the PAF-receptor-positive cells, but not in control KB cells. Similarly, treatment of the PAF-receptor-expressing primary cultures of human keratinocytes or the human epidermal cell line A-431 with carbamoyl PAF or ultraviolet B radiation resulted in interleukin-8 production that was partially inhibited by PAF receptor antagonists. These studies suggest that the epidermal PAF receptor may be a pharmacologic target for ultraviolet B radiation in skin and thus may act to augment ultraviolet-B-mediated production of cytokines such as interleukin-8.

Original languageEnglish
Pages (from-to)267-272
Number of pages6
JournalJournal of Investigative Dermatology
Volume115
Issue number2
DOIs
StatePublished - 2000

Fingerprint

Interleukin-8
Radiation
Platelet Activating Factor
KB Cells
Cytokines
Keratinocytes
Oxidative stress
Biosynthesis
Cells
platelet activating factor receptor
Oxidative Stress
Cell Line
Messenger RNA
Skin
Chemotactic Factors
Cell culture
Proteins
Chemical activation
Irradiation
Inflammation

Keywords

  • IL 8
  • Keratinocytes
  • Oxidative stress
  • Platelet-activating factor
  • UVB

ASJC Scopus subject areas

  • Dermatology

Cite this

Evidence for involvement of the epidermal platelet Activating factor receptor in ultraviolet-B-radiation induced interleukin-8 production. / Countryman, Nicholas B.; Pei, Yong; Yi, Qiaofang; Spandau, Dan; Travers, Jeffrey.

In: Journal of Investigative Dermatology, Vol. 115, No. 2, 2000, p. 267-272.

Research output: Contribution to journalArticle

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