Evidence for platelet-activating factor receptors in several B lymphoblastoid cell lines

Jeffrey Travers, James E. Shaw, Qian Li, Douglas A. Kniss, Richard H. Fertel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Previous studies have shown that Raji, an Epstein-Barr virus (EBV)-immortalized Burkitt lymphoma B cell line, contains functional platelet-activating factor (PAF) receptors. Twelve other lymphoid cell lines, including Burkitt and non-Burkitt B cell lines, T cell lines, and a non B, non T cell line were tested for the presence of PAF receptors. Radioligand binding studies conducted at 4 °C revealed that six lymphoid cell lines of B cell origin (Raji, P3HR-1, BJAB, BJA/HR-1, Dakiki and PB-1) could specifically bind [3H]PAF. Treatment of four (Raji, P3HR-1, Dakiki and PB-1) of the above lymphoid cell lines with PAF resulted in an increase in free intracellular calcium, indicating that these specific PAF binding sites were functional PAF receptors. Other B cell lines (Daudi, B95-8, sfBT, CB-1), T cell lines (MOLT-4, CCRF-CEM) and a non B, non T cell line (NALM-6) had no PAF binding sites and showed no PAF-induced increase in intracellular calcium levels. These studies demonstrate evidence for the presence of PAF receptors on several B lymphocyte cell lines.

Original languageEnglish (US)
Pages (from-to)1755-1760
Number of pages6
JournalLife Sciences
Volume49
Issue number23
DOIs
StatePublished - 1991
Externally publishedYes

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Cells
Platelet Activating Factor
Cell Line
T-cells
B-Lymphocytes
T-Lymphocytes
Lymphocytes
Binding Sites
Calcium
platelet activating factor receptor
Viruses
Burkitt Lymphoma
Human Herpesvirus 4

ASJC Scopus subject areas

  • Pharmacology

Cite this

Evidence for platelet-activating factor receptors in several B lymphoblastoid cell lines. / Travers, Jeffrey; Shaw, James E.; Li, Qian; Kniss, Douglas A.; Fertel, Richard H.

In: Life Sciences, Vol. 49, No. 23, 1991, p. 1755-1760.

Research output: Contribution to journalArticle

Travers, Jeffrey ; Shaw, James E. ; Li, Qian ; Kniss, Douglas A. ; Fertel, Richard H. / Evidence for platelet-activating factor receptors in several B lymphoblastoid cell lines. In: Life Sciences. 1991 ; Vol. 49, No. 23. pp. 1755-1760.
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