Evidence for the biclonal nature of a Waldenstrom's macroglobulinemia

Lynda Bonewald, Gabriel Virella, An Chuan Wang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The serum of a patient with Waldenstrom's macroglobulinemia was shown to contain two IgM(κ) paraproteins of different electrophoretic mobilities on cellulose acetate and immunoelectrophoresis and were designated IgM slow (s) and IgM fast (f). Both existed as high molecular weight polymers and contained a J chain. The two pentameric IgM monoclonal proteins were shown to be completely distinct in carbohydrate composition as determined by gas chromatography and periodic acid-Schiff staining, in molecular weight, by physicochemical properties, peptide mapping, amino-acid composition, NH2-terminal sequence, subgroup of light chains, and by lack of shared idiotypic determinants as determined by hemagglutination inhibition studies. Our study presents strong evidence for the 'bioclonal' nature of this gammopathy. We conclude that both paraproteins are distinct and originated from different B cells.

Original languageEnglish (US)
Pages (from-to)53-63
Number of pages11
JournalClinica Chimica Acta
Volume146
Issue number1
DOIs
StatePublished - Feb 28 1985
Externally publishedYes

Fingerprint

Waldenstrom Macroglobulinemia
Immunoglobulin M
Paraproteins
Molecular Weight
Molecular weight
Electrophoretic mobility
Immunoelectrophoresis
Periodic Acid
Peptide Mapping
Hemagglutination
Chemical analysis
Gas chromatography
Gas Chromatography
Polymers
B-Lymphocytes
Cells
Carbohydrates
Staining and Labeling
Light
Amino Acids

Keywords

  • Biclonal gammopathy
  • Immunoglobulin structure
  • Waldenstrom's macroglobulinemia

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Evidence for the biclonal nature of a Waldenstrom's macroglobulinemia. / Bonewald, Lynda; Virella, Gabriel; Wang, An Chuan.

In: Clinica Chimica Acta, Vol. 146, No. 1, 28.02.1985, p. 53-63.

Research output: Contribution to journalArticle

Bonewald, Lynda ; Virella, Gabriel ; Wang, An Chuan. / Evidence for the biclonal nature of a Waldenstrom's macroglobulinemia. In: Clinica Chimica Acta. 1985 ; Vol. 146, No. 1. pp. 53-63.
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