The uptake of [U-14C] glycine and unlabelled glycine into synaptosomes isolated from the telencephalon and spinal cord was studied. During the first 3 minutes, the rate of [U-14C] glycine (8 × 10-7M) taken up by synaptosome preparations (P2) from the spinal cord and telencephalon were estimated to be 0.100 and 0.0243 nmoles/mg protein/min, respectively. The addition of either 10-5M ouabain or 10-3M DNP significantly inhibited the accumulation of [U-14C] glycine. Approximately 85% of the radioactivity taken up by the incubated P2 fraction was found in the subfraction of P2 containing synaptosomes. A net accumulation of unlabelled glycine was observed when the crude synaptosomal fraction (P2) was incubated at 37°C in the presence of 0.0375, 0.075 and 0.15 mM glycine. In preparations from the telencephalon and spinal cord, this uptake of unlabelled glycine appeared to occur against a large concentration gradient. The rate of uptake was 2 to 6 times greater in preparations from the spinal cord than in similar preparations from the telencephalon.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)