Evidence for the net accumulation of glycine into a synaptosomal fraction isolated from the telencephalon and spinal cord of the rat

Morris H. Aprison, William J. McBride

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Abstract

The uptake of [U-14C] glycine and unlabelled glycine into synaptosomes isolated from the telencephalon and spinal cord was studied. During the first 3 minutes, the rate of [U-14C] glycine (8 × 10-7M) taken up by synaptosome preparations (P2) from the spinal cord and telencephalon were estimated to be 0.100 and 0.0243 nmoles/mg protein/min, respectively. The addition of either 10-5M ouabain or 10-3M DNP significantly inhibited the accumulation of [U-14C] glycine. Approximately 85% of the radioactivity taken up by the incubated P2 fraction was found in the subfraction of P2 containing synaptosomes. A net accumulation of unlabelled glycine was observed when the crude synaptosomal fraction (P2) was incubated at 37°C in the presence of 0.0375, 0.075 and 0.15 mM glycine. In preparations from the telencephalon and spinal cord, this uptake of unlabelled glycine appeared to occur against a large concentration gradient. The rate of uptake was 2 to 6 times greater in preparations from the spinal cord than in similar preparations from the telencephalon.

Original languageEnglish
Pages (from-to)449-458
Number of pages10
JournalLife Sciences
Volume12
Issue number10 PART 1
DOIs
StatePublished - May 15 1973

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Telencephalon
Glycine
Rats
Spinal Cord
Synaptosomes
Radioactivity
Ouabain

ASJC Scopus subject areas

  • Pharmacology

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Evidence for the net accumulation of glycine into a synaptosomal fraction isolated from the telencephalon and spinal cord of the rat. / Aprison, Morris H.; McBride, William J.

In: Life Sciences, Vol. 12, No. 10 PART 1, 15.05.1973, p. 449-458.

Research output: Contribution to journalArticle

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N2 - The uptake of [U-14C] glycine and unlabelled glycine into synaptosomes isolated from the telencephalon and spinal cord was studied. During the first 3 minutes, the rate of [U-14C] glycine (8 × 10-7M) taken up by synaptosome preparations (P2) from the spinal cord and telencephalon were estimated to be 0.100 and 0.0243 nmoles/mg protein/min, respectively. The addition of either 10-5M ouabain or 10-3M DNP significantly inhibited the accumulation of [U-14C] glycine. Approximately 85% of the radioactivity taken up by the incubated P2 fraction was found in the subfraction of P2 containing synaptosomes. A net accumulation of unlabelled glycine was observed when the crude synaptosomal fraction (P2) was incubated at 37°C in the presence of 0.0375, 0.075 and 0.15 mM glycine. In preparations from the telencephalon and spinal cord, this uptake of unlabelled glycine appeared to occur against a large concentration gradient. The rate of uptake was 2 to 6 times greater in preparations from the spinal cord than in similar preparations from the telencephalon.

AB - The uptake of [U-14C] glycine and unlabelled glycine into synaptosomes isolated from the telencephalon and spinal cord was studied. During the first 3 minutes, the rate of [U-14C] glycine (8 × 10-7M) taken up by synaptosome preparations (P2) from the spinal cord and telencephalon were estimated to be 0.100 and 0.0243 nmoles/mg protein/min, respectively. The addition of either 10-5M ouabain or 10-3M DNP significantly inhibited the accumulation of [U-14C] glycine. Approximately 85% of the radioactivity taken up by the incubated P2 fraction was found in the subfraction of P2 containing synaptosomes. A net accumulation of unlabelled glycine was observed when the crude synaptosomal fraction (P2) was incubated at 37°C in the presence of 0.0375, 0.075 and 0.15 mM glycine. In preparations from the telencephalon and spinal cord, this uptake of unlabelled glycine appeared to occur against a large concentration gradient. The rate of uptake was 2 to 6 times greater in preparations from the spinal cord than in similar preparations from the telencephalon.

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