Evidence for the presence of phospholamban in the endoplasmic reticulum of smooth muscle

Luc Raeymaekers, Larry Jones

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

In microsomal vesicles isolated from several smooth muscles many polypeptides were phosphorylated by the catalytic subunit of cyclic AMP-dependent protein kinase. In pig stomach and in rabbit and dog aorta components of Mr 22 000 and 11 000 were identified as forms of phospholamban. These polypeptides were, however, not observed in pig aorta. These phospholamban-like polypeptides presented the same electrophoretic mobility in sodium dodecyl sulphate gels as cardiac phospholamban, and the 22 000 Mr form showed a similar reaction to heat treatment in sodium dodecyl sulphate. Antibodies against purified canine cardiac phospholamban cross-reacted with the 22 000 and 11 000 Mr phosphorylatable polypeptides from smooth muscle membranes. Subcellular fractionation of porcine stomach smooth muslce indicated that phospholamban was present in the membranes of the endoplasmic reticulum and not in the plasma membranes. Phospholamban was also phosphorylated by an endogenous calcium-calmodulin-dependent protein kinase and by an endogenous cyclic AMP-dependent kinase. It is concluded that the endoplasmic reticulum of many, but possibly not all, smooth muscles contains phospholamban. However, the physiological role of phospholamban in smooth muscle remains to be established.

Original languageEnglish
Pages (from-to)258-265
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume882
Issue number2
DOIs
StatePublished - Jun 19 1986

Fingerprint

Endoplasmic Reticulum
Smooth Muscle
Muscle
Peptides
Swine
Sodium Dodecyl Sulfate
Aorta
Stomach
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
Membranes
Adenylate Kinase
Electrophoretic mobility
Calcium-Calmodulin-Dependent Protein Kinases
phospholamban
Cell membranes
Fractionation
Cyclic AMP
Canidae
Phosphotransferases
Hot Temperature

Keywords

  • (Smooth muscle)
  • Phospholamban
  • Protein phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Evidence for the presence of phospholamban in the endoplasmic reticulum of smooth muscle. / Raeymaekers, Luc; Jones, Larry.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 882, No. 2, 19.06.1986, p. 258-265.

Research output: Contribution to journalArticle

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