Evidence for transformation of fibroadenoma of the breast to malignant phyllodes tumor

Kurt B. Hodges, Fadi W. Abdul-Karim, Mingsheng Wang, Antonio Lopez-Beltran, Rodolfo Montironi, Samantha Easley, Shaobo Zhang, Nancy Wang, Gregory T. MacLennan, Liang Cheng

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Fibroadenoma (FA) and phyllodes tumor (PT) of the breast are biphasic tumors composed of variable proportions of epithelial and stromal cells. We identified a case of synchronous FA and PT in the same breast mass. Using laser capture microdissection, loss of heterozygosity analysis was performed on these components to gain potential insight into the histogenetic relationship between FA and PT. Genomic DNA was analyzed at 10 microsatellite loci by polymerase chain reaction. Both tumors showed allelic loss at D7S522. In addition, phylloid tumor showed allelic loss at TP53 and D22S264, not observed in FA. Our data suggest that FA and PT are clonally related and allelic loss at TP53 and D22S264 may be implicated in the progression of FA to phyllode tumor.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalApplied Immunohistochemistry and Molecular Morphology
Volume17
Issue number4
DOIs
StatePublished - Jul 2009

Fingerprint

Phyllodes Tumor
Fibroadenoma
Breast
Loss of Heterozygosity
Laser Capture Microdissection
Stromal Cells
Microsatellite Repeats
Neoplasms
Epithelial Cells
Breast Neoplasms
Polymerase Chain Reaction
DNA

Keywords

  • Breast
  • Cancer progression
  • Clonality
  • Fibroadenoma
  • Laser capture microdissection
  • Molecular genetics
  • Phyllodes tumor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology
  • Histology

Cite this

Evidence for transformation of fibroadenoma of the breast to malignant phyllodes tumor. / Hodges, Kurt B.; Abdul-Karim, Fadi W.; Wang, Mingsheng; Lopez-Beltran, Antonio; Montironi, Rodolfo; Easley, Samantha; Zhang, Shaobo; Wang, Nancy; MacLennan, Gregory T.; Cheng, Liang.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 17, No. 4, 07.2009, p. 345-350.

Research output: Contribution to journalArticle

Hodges, Kurt B. ; Abdul-Karim, Fadi W. ; Wang, Mingsheng ; Lopez-Beltran, Antonio ; Montironi, Rodolfo ; Easley, Samantha ; Zhang, Shaobo ; Wang, Nancy ; MacLennan, Gregory T. ; Cheng, Liang. / Evidence for transformation of fibroadenoma of the breast to malignant phyllodes tumor. In: Applied Immunohistochemistry and Molecular Morphology. 2009 ; Vol. 17, No. 4. pp. 345-350.
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abstract = "Fibroadenoma (FA) and phyllodes tumor (PT) of the breast are biphasic tumors composed of variable proportions of epithelial and stromal cells. We identified a case of synchronous FA and PT in the same breast mass. Using laser capture microdissection, loss of heterozygosity analysis was performed on these components to gain potential insight into the histogenetic relationship between FA and PT. Genomic DNA was analyzed at 10 microsatellite loci by polymerase chain reaction. Both tumors showed allelic loss at D7S522. In addition, phylloid tumor showed allelic loss at TP53 and D22S264, not observed in FA. Our data suggest that FA and PT are clonally related and allelic loss at TP53 and D22S264 may be implicated in the progression of FA to phyllode tumor.",
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AU - Wang, Mingsheng

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AU - Montironi, Rodolfo

AU - Easley, Samantha

AU - Zhang, Shaobo

AU - Wang, Nancy

AU - MacLennan, Gregory T.

AU - Cheng, Liang

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KW - Cancer progression

KW - Clonality

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