Abstract
To determine whether the neurogenetic patterns of Purkinje cells and deep cerebellar nuclei neurons were normal in weaver homozygotes and whether the degeneration of those neuronal types was linked to their time of origin, [3H] thymidine autoradiography was applied on sections of homozygous weaver mice and normal controls on postnatal day 90. The experimental animals were the offspring of pregnant dams injected with [3H] thymidine on embryonic days 11-12, 12-13, 13-14 and 14-15. The results show that the onset of neurogenesis, its pattern of peaks and valleys, and its total span were similar between wild type and homozygous weaver in the cerebellar areas analyzed, indicating that the loss of Purkinje cells and deep cerebellar nuclei neurons is not related to neurogenetic patterns. In weaver homozygotes, the loss of Purkinje cells and deep cerebellar nuclei neurons followed a lateral to medial gradient of increasing severity. Thus, the vermis and the fastigial nucleus, which are medially located, presented the most important neuron loss, whereas in the lateral hemisphere and the dentate nucleus, neuron loss was spared.
| Original language | English |
|---|---|
| Pages (from-to) | 599-610 |
| Number of pages | 12 |
| Journal | International Journal of Developmental Neuroscience |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2001 |
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Keywords
- [H] thymidine autoradiography
- Cerebellar cortex
- Deep cerebellar nuclei neurons
- Neurogenetic patterns
- Purkinje cells
- Weaver gene
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
Evidence that the loss of Purkinje cells and deep cerebellar nuclei neurons in homozygous weaver is not related to neurogenetic patterns. / Martí, Joaquín; Wills, Katherine V.; Ghetti, Bernardino; Bayer, Shirley A.
In: International Journal of Developmental Neuroscience, Vol. 19, No. 6, 2001, p. 599-610.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence that the loss of Purkinje cells and deep cerebellar nuclei neurons in homozygous weaver is not related to neurogenetic patterns
AU - Martí, Joaquín
AU - Wills, Katherine V.
AU - Ghetti, Bernardino
AU - Bayer, Shirley A.
PY - 2001
Y1 - 2001
N2 - To determine whether the neurogenetic patterns of Purkinje cells and deep cerebellar nuclei neurons were normal in weaver homozygotes and whether the degeneration of those neuronal types was linked to their time of origin, [3H] thymidine autoradiography was applied on sections of homozygous weaver mice and normal controls on postnatal day 90. The experimental animals were the offspring of pregnant dams injected with [3H] thymidine on embryonic days 11-12, 12-13, 13-14 and 14-15. The results show that the onset of neurogenesis, its pattern of peaks and valleys, and its total span were similar between wild type and homozygous weaver in the cerebellar areas analyzed, indicating that the loss of Purkinje cells and deep cerebellar nuclei neurons is not related to neurogenetic patterns. In weaver homozygotes, the loss of Purkinje cells and deep cerebellar nuclei neurons followed a lateral to medial gradient of increasing severity. Thus, the vermis and the fastigial nucleus, which are medially located, presented the most important neuron loss, whereas in the lateral hemisphere and the dentate nucleus, neuron loss was spared.
AB - To determine whether the neurogenetic patterns of Purkinje cells and deep cerebellar nuclei neurons were normal in weaver homozygotes and whether the degeneration of those neuronal types was linked to their time of origin, [3H] thymidine autoradiography was applied on sections of homozygous weaver mice and normal controls on postnatal day 90. The experimental animals were the offspring of pregnant dams injected with [3H] thymidine on embryonic days 11-12, 12-13, 13-14 and 14-15. The results show that the onset of neurogenesis, its pattern of peaks and valleys, and its total span were similar between wild type and homozygous weaver in the cerebellar areas analyzed, indicating that the loss of Purkinje cells and deep cerebellar nuclei neurons is not related to neurogenetic patterns. In weaver homozygotes, the loss of Purkinje cells and deep cerebellar nuclei neurons followed a lateral to medial gradient of increasing severity. Thus, the vermis and the fastigial nucleus, which are medially located, presented the most important neuron loss, whereas in the lateral hemisphere and the dentate nucleus, neuron loss was spared.
KW - [H] thymidine autoradiography
KW - Cerebellar cortex
KW - Deep cerebellar nuclei neurons
KW - Neurogenetic patterns
KW - Purkinje cells
KW - Weaver gene
UR - http://www.scopus.com/inward/record.url?scp=0034801266&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034801266&partnerID=8YFLogxK
U2 - 10.1016/S0736-5748(01)00036-3
DO - 10.1016/S0736-5748(01)00036-3
M3 - Article
C2 - 11600321
AN - SCOPUS:0034801266
VL - 19
SP - 599
EP - 610
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
SN - 0736-5748
IS - 6
ER -