Evidence that trypsin digestion exposes a channel in the sarcoplasmic reticulum membrane

K. C. Toogood, B. Folsom, T. Topping, H. McCutchan, M. J. Dolejsi, S. Johns, G. Stuart, A. Dunker

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We present evidence that proteolytic digestion of sarcoplasmic reticulum membranes with trypsin exposes an ion-ophore that is capable of translocating calcium across the membrane of preloaded vesicles. Net transport of calcium appears to stop when the chemical potential of the ion on both sides of the membrane is equal. The temperature coefficient of steady-state leakage suggests that the ionophore is of the channel or pore type. We suggest that tryptic digestion exposes a channel in sarcoplasmic reticulum membranes through which calcium, and perhaps other ions as well, can diffuse down concentration gradients.

Original languageEnglish (US)
Pages (from-to)49-75
Number of pages27
JournalMolecular Membrane Biology
Volume5
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Sarcoplasmic Reticulum
Trypsin
Digestion
Membranes
Ions
Calcium
Chemical potential
Ionophores
Temperature

Keywords

  • Calcium efflux
  • Sarcoplasmic ReticulumIonop-hore

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Cite this

Evidence that trypsin digestion exposes a channel in the sarcoplasmic reticulum membrane. / Toogood, K. C.; Folsom, B.; Topping, T.; McCutchan, H.; Dolejsi, M. J.; Johns, S.; Stuart, G.; Dunker, A.

In: Molecular Membrane Biology, Vol. 5, No. 1, 1983, p. 49-75.

Research output: Contribution to journalArticle

Toogood, KC, Folsom, B, Topping, T, McCutchan, H, Dolejsi, MJ, Johns, S, Stuart, G & Dunker, A 1983, 'Evidence that trypsin digestion exposes a channel in the sarcoplasmic reticulum membrane', Molecular Membrane Biology, vol. 5, no. 1, pp. 49-75. https://doi.org/10.3109/09687688309149633
Toogood, K. C. ; Folsom, B. ; Topping, T. ; McCutchan, H. ; Dolejsi, M. J. ; Johns, S. ; Stuart, G. ; Dunker, A. / Evidence that trypsin digestion exposes a channel in the sarcoplasmic reticulum membrane. In: Molecular Membrane Biology. 1983 ; Vol. 5, No. 1. pp. 49-75.
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AU - Stuart, G.

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