Evolution of the cutaneous immune response to experimental Haemophilus ducreyi infection and its relevance to HIV-1 acquisition

Tricia L. Humphreys, Carol T. Schnizlein-Bick, Barry Katz, Lee Ann Baldridge, Antoinette F. Hood, Robert A. Hromas, Stanley Spinola

Research output: Contribution to journalArticle

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Abstract

Haemophilus ducreyi causes the sexually transmitted disease chancroid, which facilitates HIV-1 transmission. Skin biopsies were obtained from subjects experimentally infected with H. ducreyi to study the evolution of the immune response and immunophenotypes relevant to transmission of HIV-1. Compared with peripheral blood, there was an enrichment of T cells and macrophages after 48 h of infection in the skin. Neutrophils became the predominant cell type by 7-9 days. By immunohistochemistry, macrophage-inflammatory protein-1α was not present early in infection, but was abundant at later stages. RANTES was present throughout the papular and pustular stages of experimental infection, but not present in uninfected control skin. Stromal cell-derived factor-1 was present at low levels in all samples examined. Macrophages in lesions had significantly increased expression of CCR5 and CXCR4 compared with peripheral blood cells, and CD4 T cells had significant up-regulation of CCR5. The magnitude of increased expression of these receptors was not replicated when PBMCs were incubated with H. ducreyi or H. ducreyi lipooligosaccharide in vitro. Together with the disruption of mucosal and skin barriers, the presence of cells with up-regulated HIV-1 coreceptors in H. ducreyi-infected lesions may provide an environment that facilitates the acquisition of R5 (CCR5), X4 (CXCR4), and dual-tropic HIV-1 strains.

Original languageEnglish
Pages (from-to)6316-6323
Number of pages8
JournalJournal of Immunology
Volume169
Issue number11
StatePublished - Dec 1 2002

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Haemophilus ducreyi
Haemophilus Infections
HIV-1
Skin
Infection
Chancroid
Macrophages
T-Lymphocytes
Macrophage Inflammatory Proteins
Chemokine CXCL12
Chemokine CCL5
Sexually Transmitted Diseases
Blood Cells
Neutrophils
Up-Regulation
Immunohistochemistry
Biopsy

ASJC Scopus subject areas

  • Immunology

Cite this

Humphreys, T. L., Schnizlein-Bick, C. T., Katz, B., Baldridge, L. A., Hood, A. F., Hromas, R. A., & Spinola, S. (2002). Evolution of the cutaneous immune response to experimental Haemophilus ducreyi infection and its relevance to HIV-1 acquisition. Journal of Immunology, 169(11), 6316-6323.

Evolution of the cutaneous immune response to experimental Haemophilus ducreyi infection and its relevance to HIV-1 acquisition. / Humphreys, Tricia L.; Schnizlein-Bick, Carol T.; Katz, Barry; Baldridge, Lee Ann; Hood, Antoinette F.; Hromas, Robert A.; Spinola, Stanley.

In: Journal of Immunology, Vol. 169, No. 11, 01.12.2002, p. 6316-6323.

Research output: Contribution to journalArticle

Humphreys, TL, Schnizlein-Bick, CT, Katz, B, Baldridge, LA, Hood, AF, Hromas, RA & Spinola, S 2002, 'Evolution of the cutaneous immune response to experimental Haemophilus ducreyi infection and its relevance to HIV-1 acquisition', Journal of Immunology, vol. 169, no. 11, pp. 6316-6323.
Humphreys TL, Schnizlein-Bick CT, Katz B, Baldridge LA, Hood AF, Hromas RA et al. Evolution of the cutaneous immune response to experimental Haemophilus ducreyi infection and its relevance to HIV-1 acquisition. Journal of Immunology. 2002 Dec 1;169(11):6316-6323.
Humphreys, Tricia L. ; Schnizlein-Bick, Carol T. ; Katz, Barry ; Baldridge, Lee Ann ; Hood, Antoinette F. ; Hromas, Robert A. ; Spinola, Stanley. / Evolution of the cutaneous immune response to experimental Haemophilus ducreyi infection and its relevance to HIV-1 acquisition. In: Journal of Immunology. 2002 ; Vol. 169, No. 11. pp. 6316-6323.
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