Evolution of the Drosophila broad locus: The Manduca sexta broad Z4 isoform has biological activity in Drosophila

Cynthia Bayer, Xiaofeng Zhou, Baohua Zhou, Lynn M. Riddiford, Laurence von Kalm

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The Drosophila melanogaster broad locus is essential for normal metamorphic development. Broad encodes three genetically distinct functions (rbp, br, and 2Bc) and a family of four zinc-finger DNA-binding proteins (Z1-Z4). The Z1, Z2, and Z3 protein isoforms are primarily associated with the rbp, br, and 2Bc genetic functions respectively. The Z4 protein isoform also provides some rbp genetic function, however an essential function for the Z4 isoform in metamorphosis has not been identified. To determine the degree of conservation of Z4 function between the tobacco hornworm Manduca sexta and Drosophila we generated transgenic Drosophila expressing the Manduca broad Z4 isoform and used this transgene to rescue rbp mutant lethality during Drosophila metamorphosis. We find that the Manduca Z4 protein has significant biological activity in Drosophila with respect to rescue of rbp-associated lethality. There was also some overlap in effects on cuticle gene expression between the Manduca Z4 and Drosophila Z1 isoforms that was not shared with the Drosophila Z4 isoform. Our findings show that Z4 function has been conserved over the 260-million-year period since the divergence of Diptera and Lepidoptera, and are consistent with the hypothesis that the Drosophila Z4 and Manduca Z4 isoforms have essential roles in metamorphosis.

Original languageEnglish (US)
Pages (from-to)471-476
Number of pages6
JournalDevelopment Genes and Evolution
Volume213
Issue number10
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Manduca
Manduca sexta
Drosophila
bioactive properties
Protein Isoforms
metamorphosis
loci
protein
protein isoforms
cuticle
tobacco
gene expression
zinc
divergence
Lepidoptera
DNA-binding proteins
DNA
zinc finger motif
Zinc Fingers
DNA-Binding Proteins

Keywords

  • BR-C
  • Broad-Complex
  • Ecdysone
  • Gene duplication
  • Metamorphosis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Evolution of the Drosophila broad locus : The Manduca sexta broad Z4 isoform has biological activity in Drosophila. / Bayer, Cynthia; Zhou, Xiaofeng; Zhou, Baohua; Riddiford, Lynn M.; von Kalm, Laurence.

In: Development Genes and Evolution, Vol. 213, No. 10, 10.2003, p. 471-476.

Research output: Contribution to journalArticle

Bayer, Cynthia ; Zhou, Xiaofeng ; Zhou, Baohua ; Riddiford, Lynn M. ; von Kalm, Laurence. / Evolution of the Drosophila broad locus : The Manduca sexta broad Z4 isoform has biological activity in Drosophila. In: Development Genes and Evolution. 2003 ; Vol. 213, No. 10. pp. 471-476.
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