Ex vivo culture of mouse skin activates an interleukin 1 alpha-dependent inflammatory response

Hong Ming Zhou, Radomir M. Slominski, Leroy J. Seymour, Maria C. Bell, Priya Dave, Joseph Atumonye, William Wright, Avery Dawes, Brad Griesenauer, Sophie Paczesny, Mark H. Kaplan, Dan F. Spandau, Matthew J. Turner

Research output: Contribution to journalArticle

Abstract

Ex vivo culture of mouse and human skin causes an inflammatory response characterized by production of multiple cytokines. We used ex vivo culture of mouse tail skin specimens to investigate mechanisms of this skin culture-induced inflammatory response. Multiplex assays revealed production of interleukin 1 alpha (IL-1α), interleukin 1 beta (IL-1β), interleukin 6 (IL-6), chemokine C-X-C motif ligand 1 (CXCL1), granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) during skin culture, and quantitative PCR revealed transcripts for these proteins were also increased. Ex vivo cultures of skin from myeloid differentiation primary response 88 deficient mice (Myd88−/−) demonstrated significantly reduced expression of transcripts for the aforementioned cytokines. The same result was observed with skin from interleukin 1 receptor type 1 deficient mice (Il1r1−/−). These data suggested the IL-1R1/MyD88 axis is required for the skin culture-induced inflammatory response and led us to investigate the role of IL-1α and IL-1β (the ligands for IL-1R1) in this process. Addition of IL-1α neutralizing antibody to skin cultures significantly reduced expression of Cxcl1, Il6 and Csf3. IL-1β neutralization did not reduce levels of these transcripts. These studies suggest that IL-1α promotes the skin the culture-induced inflammatory response.

Original languageEnglish (US)
JournalExperimental Dermatology
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Interleukin-1alpha
Skin
Chemokine CXCL1
Interleukin-1 Type I Receptors
Cytokines
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Neutralizing Antibodies
Interleukin-1beta
Tail
Interleukin-6
Assays
Ligands
Polymerase Chain Reaction

Keywords

  • cytokines
  • explant
  • IL-1α
  • keratinocytes
  • mice

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Zhou, H. M., Slominski, R. M., Seymour, L. J., Bell, M. C., Dave, P., Atumonye, J., ... Turner, M. J. (Accepted/In press). Ex vivo culture of mouse skin activates an interleukin 1 alpha-dependent inflammatory response. Experimental Dermatology. https://doi.org/10.1111/exd.14044

Ex vivo culture of mouse skin activates an interleukin 1 alpha-dependent inflammatory response. / Zhou, Hong Ming; Slominski, Radomir M.; Seymour, Leroy J.; Bell, Maria C.; Dave, Priya; Atumonye, Joseph; Wright, William; Dawes, Avery; Griesenauer, Brad; Paczesny, Sophie; Kaplan, Mark H.; Spandau, Dan F.; Turner, Matthew J.

In: Experimental Dermatology, 01.01.2019.

Research output: Contribution to journalArticle

Zhou, HM, Slominski, RM, Seymour, LJ, Bell, MC, Dave, P, Atumonye, J, Wright, W, Dawes, A, Griesenauer, B, Paczesny, S, Kaplan, MH, Spandau, DF & Turner, MJ 2019, 'Ex vivo culture of mouse skin activates an interleukin 1 alpha-dependent inflammatory response', Experimental Dermatology. https://doi.org/10.1111/exd.14044
Zhou, Hong Ming ; Slominski, Radomir M. ; Seymour, Leroy J. ; Bell, Maria C. ; Dave, Priya ; Atumonye, Joseph ; Wright, William ; Dawes, Avery ; Griesenauer, Brad ; Paczesny, Sophie ; Kaplan, Mark H. ; Spandau, Dan F. ; Turner, Matthew J. / Ex vivo culture of mouse skin activates an interleukin 1 alpha-dependent inflammatory response. In: Experimental Dermatology. 2019.
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