Ex Vivo Fat Graft Preservation

Effects and Implications of Cryopreservation

Julia W. MacRae, Sunil S. Tholpady, Roy C. Ogle, Raymond F. Morgan, James H. Carraway, Henry C. Vasconez

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

There are a variety of recommended methods for harvesting, treating, and utilizing autologous fat grafts. Previous work with the MTT assay illustrated that various preimplantation handling techniques had minimal effect on the viability of fat samples. This assay was used to test the viability of harvested fat samples after being stored for up to 8 days in a variety of conditions. Surprisingly, freezing the fat before assaying also had no measurable detrimental effect, which led us to study this phenomenon in greater detail. The results demonstrated that fat stored at subzero temperatures showed remarkable maintenance of their mitochondrial metabolic activity as compared with fat stored in a 32°C incubator. These data suggest exciting possibilities for storage and banking of human adipose tissue, which would reduce patient cost, discomfort, and time associated with multiple grafting procedures.

Original languageEnglish (US)
Pages (from-to)281-283
Number of pages3
JournalAnnals of Plastic Surgery
Volume52
Issue number3
DOIs
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Cryopreservation
Fats
Transplants
Incubators
Freezing
Adipose Tissue
Maintenance
Costs and Cost Analysis
Temperature

ASJC Scopus subject areas

  • Surgery

Cite this

MacRae, J. W., Tholpady, S. S., Ogle, R. C., Morgan, R. F., Carraway, J. H., & Vasconez, H. C. (2004). Ex Vivo Fat Graft Preservation: Effects and Implications of Cryopreservation. Annals of Plastic Surgery, 52(3), 281-283. https://doi.org/10.1097/01.sap.0000110559.58945.2e

Ex Vivo Fat Graft Preservation : Effects and Implications of Cryopreservation. / MacRae, Julia W.; Tholpady, Sunil S.; Ogle, Roy C.; Morgan, Raymond F.; Carraway, James H.; Vasconez, Henry C.

In: Annals of Plastic Surgery, Vol. 52, No. 3, 03.2004, p. 281-283.

Research output: Contribution to journalArticle

MacRae, JW, Tholpady, SS, Ogle, RC, Morgan, RF, Carraway, JH & Vasconez, HC 2004, 'Ex Vivo Fat Graft Preservation: Effects and Implications of Cryopreservation', Annals of Plastic Surgery, vol. 52, no. 3, pp. 281-283. https://doi.org/10.1097/01.sap.0000110559.58945.2e
MacRae, Julia W. ; Tholpady, Sunil S. ; Ogle, Roy C. ; Morgan, Raymond F. ; Carraway, James H. ; Vasconez, Henry C. / Ex Vivo Fat Graft Preservation : Effects and Implications of Cryopreservation. In: Annals of Plastic Surgery. 2004 ; Vol. 52, No. 3. pp. 281-283.
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