Ex vivo rapamycin treatment of human cord blood CD34+ cells enhances their engraftment of NSG mice

Sara L. Rohrabaugh, Timothy B. Campbell, Giao Hangoc, Hal Broxmeyer

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Since cord blood (CB) has become a commonly used source of transplantable hematopoietic stem (HSC) and hematopoietic progenitor cells (HPC), there has been a need to overcome the limited HSC and HPC numbers available to transplant from a single CB, especially for adult recipients. Our laboratory previously demonstrated that Rheb2 overexpression significantly impaired the repopulating ability of HSC. Since overexpression of Rheb2 leads to increased signaling through mTOR, we examined the effect of the mTOR inhibitor rapamycin ex vivo on cytokine expanded CD34+ CB cells for the engraftment of these cells in non-obese diabetic, severe combined immunodeficient, IL-2 receptor γ chain null (NSG) mice. We observed significant enhancement in engraftment of the CB treated ex vivo with cytokines in the presence of rapamycin prior to transplant, effects seen in primary as well as secondary transplants. These pre-clinical results suggest a positive role for rapamycin during ex vivo culture of CB SCID repopulating cells/HSC.

Original languageEnglish
Pages (from-to)318-320
Number of pages3
JournalBlood Cells, Molecules and Diseases
Volume46
Issue number4
DOIs
StatePublished - Apr 15 2011

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Sirolimus
Fetal Blood
Blood Cells
Hematopoietic Stem Cells
Transplants
Cytokines
Interleukin-2 Receptors
Cell Count

Keywords

  • CD34 cell engraftment
  • Cord blood
  • Ex vivo expansion
  • MTOR
  • Rapamycin

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology
  • Cell Biology

Cite this

Ex vivo rapamycin treatment of human cord blood CD34+ cells enhances their engraftment of NSG mice. / Rohrabaugh, Sara L.; Campbell, Timothy B.; Hangoc, Giao; Broxmeyer, Hal.

In: Blood Cells, Molecules and Diseases, Vol. 46, No. 4, 15.04.2011, p. 318-320.

Research output: Contribution to journalArticle

Rohrabaugh, Sara L. ; Campbell, Timothy B. ; Hangoc, Giao ; Broxmeyer, Hal. / Ex vivo rapamycin treatment of human cord blood CD34+ cells enhances their engraftment of NSG mice. In: Blood Cells, Molecules and Diseases. 2011 ; Vol. 46, No. 4. pp. 318-320.
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