Examination of the internal promoter, Pe, in the ilvGMEDA operon of E. coli K-12

Ronald Wek, G. Wesley Hatfield

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The ilvGMEDA operon of Escherichia coli K-12 contains an internal promoter, PE, in the distal portion of the ilvM gene immediately upstream from the ilvE gene. The location of this promoter was determined using S1 nuclease protection analyses of in vivo and in vitro transcripts. The transcriptional activity of the internal promoter was compared to the transcriptional activity of the operon-proximal promoter P1P2 using transcriptional fusion vectors and plasmid copy number determinations. These measurements showed that the P1P2 promoter is 52-fold stronger than the internal PE promoter. Estimates of the transcriptional role of the internal promoter on ilvE gene expression during growth conditions in excess and limiting branch chain amino acids is presented.

Original languageEnglish (US)
Pages (from-to)2763-2777
Number of pages15
JournalNucleic Acids Research
Volume14
Issue number6
DOIs
StatePublished - Mar 11 1986
Externally publishedYes

Fingerprint

Operon
Promoter
Escherichia coli
Escherichia Coli
Genes
Internal
Gene expression
Amino acids
Plasmids
Fusion reactions
Gene Expression
Amino Acids
Growth
Gene
Growth Conditions
Excess
Immediately
Fusion
Branch
Fold

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Examination of the internal promoter, Pe, in the ilvGMEDA operon of E. coli K-12. / Wek, Ronald; Hatfield, G. Wesley.

In: Nucleic Acids Research, Vol. 14, No. 6, 11.03.1986, p. 2763-2777.

Research output: Contribution to journalArticle

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