Excitation-contraction uncoupling during ischemia in the blood perfused dog heart

Marc J. Levine, Kazumasa Harada, Arthur J. Meuse, Jun Watanabe, Jeffrey Breall, Joseph P. Carrozza, Lisa Bentivegna, Alvin Franklin, Robert G. Johnson, William Grossman, James P. Morgan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The bioluminescent Ca2+-indicator, aequorin, was loaded into the left ventricular apex of blood-perfused hearts from 13 dogs for simultaneous recording of left ventricular pressure and intracellular calcium levels. During a 2 minute period of ischemia, systolic and diastolic pressures significantly decreased. In contrast, these pressure changes were associated with an increase in both systolic and diastolic calcium reaching a maximum diastolic value of 0.59 μM and a systolic value of 1.11 μM. This apparent dissociation between pressure and [Ca2+]i supports the hypothesis that changes in myofilament Ca2+ responsiveness are of major importance in modulating contractility during ischemia in large mammalian hearts.

Original languageEnglish (US)
Pages (from-to)502-506
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number1
DOIs
StatePublished - Aug 30 1991
Externally publishedYes

Fingerprint

Blood
Ischemia
Aequorin
Dogs
Blood Pressure
Calcium
Pressure
Myofibrils
Ventricular Pressure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Excitation-contraction uncoupling during ischemia in the blood perfused dog heart. / Levine, Marc J.; Harada, Kazumasa; Meuse, Arthur J.; Watanabe, Jun; Breall, Jeffrey; Carrozza, Joseph P.; Bentivegna, Lisa; Franklin, Alvin; Johnson, Robert G.; Grossman, William; Morgan, James P.

In: Biochemical and Biophysical Research Communications, Vol. 179, No. 1, 30.08.1991, p. 502-506.

Research output: Contribution to journalArticle

Levine, MJ, Harada, K, Meuse, AJ, Watanabe, J, Breall, J, Carrozza, JP, Bentivegna, L, Franklin, A, Johnson, RG, Grossman, W & Morgan, JP 1991, 'Excitation-contraction uncoupling during ischemia in the blood perfused dog heart', Biochemical and Biophysical Research Communications, vol. 179, no. 1, pp. 502-506. https://doi.org/10.1016/0006-291X(91)91399-W
Levine, Marc J. ; Harada, Kazumasa ; Meuse, Arthur J. ; Watanabe, Jun ; Breall, Jeffrey ; Carrozza, Joseph P. ; Bentivegna, Lisa ; Franklin, Alvin ; Johnson, Robert G. ; Grossman, William ; Morgan, James P. / Excitation-contraction uncoupling during ischemia in the blood perfused dog heart. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 179, No. 1. pp. 502-506.
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