Excitation-contraction uncoupling during ischemia in the blood perfused dog heart

Marc J. Levine, Kazumasa Harada, Arthur J. Meuse, Jun Watanabe, Jeffrey Breall, Joseph P. Carrozza, Lisa Bentivegna, Alvin Franklin, Robert G. Johnson, William Grossman, James P. Morgan

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Abstract

The bioluminescent Ca2+-indicator, aequorin, was loaded into the left ventricular apex of blood-perfused hearts from 13 dogs for simultaneous recording of left ventricular pressure and intracellular calcium levels. During a 2 minute period of ischemia, systolic and diastolic pressures significantly decreased. In contrast, these pressure changes were associated with an increase in both systolic and diastolic calcium reaching a maximum diastolic value of 0.59 μM and a systolic value of 1.11 μM. This apparent dissociation between pressure and [Ca2+]i supports the hypothesis that changes in myofilament Ca2+ responsiveness are of major importance in modulating contractility during ischemia in large mammalian hearts.

Original languageEnglish (US)
Pages (from-to)502-506
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number1
DOIs
StatePublished - Aug 30 1991

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Levine, M. J., Harada, K., Meuse, A. J., Watanabe, J., Breall, J., Carrozza, J. P., Bentivegna, L., Franklin, A., Johnson, R. G., Grossman, W., & Morgan, J. P. (1991). Excitation-contraction uncoupling during ischemia in the blood perfused dog heart. Biochemical and Biophysical Research Communications, 179(1), 502-506. https://doi.org/10.1016/0006-291X(91)91399-W