Excitatory monocyte chemoattractant protein-1 signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion

Fletcher White, Jihu Sun, Stephen M. Waters, Chao Ma, Dongjun Ren, Matthew Ripsch, Jeremy Steflik, Daniel N. Cortright, Robert H. LaMotte, Richard J. Miller

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

Neuronal hyperexcitability in both injured and adjacent uninjured neurons is associated with states of chronic injury and pain and is likely subject to neuroinflammatory processes. Chronic inflammatory responses are largely orchestrated by chemokines. One chemokine, monocyte chemoattractant protein-1 (MCP-1), in the presence of its cognate receptor, the β chemokine receptor 2 (CCR2), produces neural activity in dissociated neuronal cultures of neonatal dorsal root ganglion (DRG) neurons. Using a neuropathic pain model, chronic compression of the DRG (CCD), we compared anatomically separate populations of noncompressed lumbar DRG (L3/L6) with compressed lumbar DRG (L4/L5) for changes in the gene expression of CCR2. In situ hybridization revealed that CCR2 mRNA was up-regulated in neurons and nonneuronal cells present in both compressed L4/L5 and ipsilateral noncompressed L3/L6 DRGs at postoperative day 5 (POD5). The total percentages of compressed and noncompressed neurons exhibiting CCR2 mRNA transcripts in L3, L5, and L6 DRG were 33 ± 3.5%, 49 ± 6.2%, and 41 ± 5.6%, respectively, and included cell bodies of small, medium, and large size. In addition, the preferred CCR2 ligand, MCP-1, was up-regulated by POD5 in both compressed L4/L5 and noncompressed L3/L6 DRG neurons. Application of MCP-1 to the cell bodies of the intact formerly compressed DRG in vitro produced potent excitatory effects not observed in control ganglia, MCP-1/CCR2 signaling is directly involved with a chronic compression injury and may contribute to associated neuronal hyperexcitability and neuropathic pain.

Original languageEnglish (US)
Pages (from-to)14092-14097
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number39
DOIs
StatePublished - Sep 27 2005
Externally publishedYes

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Chemokine CCL2
Spinal Ganglia
Sensory Receptor Cells
Chemokine Receptors
Neurons
Neuralgia
Chemokines
CCR2 Receptors
Messenger RNA
Diagnosis-Related Groups
Wounds and Injuries
Ganglia
Chronic Pain
In Situ Hybridization
Ligands
Gene Expression
Population

Keywords

  • Hyperalgesia
  • Nerve injury
  • Neuropathic pain
  • Peripheral sensitization

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Excitatory monocyte chemoattractant protein-1 signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion. / White, Fletcher; Sun, Jihu; Waters, Stephen M.; Ma, Chao; Ren, Dongjun; Ripsch, Matthew; Steflik, Jeremy; Cortright, Daniel N.; LaMotte, Robert H.; Miller, Richard J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 39, 27.09.2005, p. 14092-14097.

Research output: Contribution to journalArticle

White, Fletcher ; Sun, Jihu ; Waters, Stephen M. ; Ma, Chao ; Ren, Dongjun ; Ripsch, Matthew ; Steflik, Jeremy ; Cortright, Daniel N. ; LaMotte, Robert H. ; Miller, Richard J. / Excitatory monocyte chemoattractant protein-1 signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 39. pp. 14092-14097.
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AU - Sun, Jihu

AU - Waters, Stephen M.

AU - Ma, Chao

AU - Ren, Dongjun

AU - Ripsch, Matthew

AU - Steflik, Jeremy

AU - Cortright, Daniel N.

AU - LaMotte, Robert H.

AU - Miller, Richard J.

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