Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34+ cells

Yasuhiro Ebihara, Ming Jiang Xu, Atsushi Manabe, Akira Kikuchi, Ryuhei Tanaka, Shunichi Kato, Tatsutoshi Nakahata, Kohichiro Tsuji

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Although granulocyte colony-stimulating factor (G-CSF) has been reported to act on cells of neutrophilic lineage, the administration of G-CSF to induce the mobilization of various haematopoietic progenitors into the circulation. We analysed the expression of receptors for G-CSF (G-CSFR) on human bone marrow and G-CSF-mobilized peripheral blood CD34+ cells, and examined the proliferation and differentiation capabilities of sorted CD34+G-CSFR+ and CD34+G-CSFR- cells using methylcellulose clonal culture. Flow cytometric analysis showed that G-CSFR was expressed on 14.9 ± 4.9% of bone marrow CD34+ cells, most of which were included in CD34+CD33+ and CD34+CD38+ cell fractions. In clonal cultures, CD34+G-CSFR+ cells produced only myeloid colonies, whereas CD34+G-CSFR- cells produced erythroid bursts, megakaryocyte and multilineage colonies. When incubated with the cytokine cocktail for 5 d, CD34+G-CSFR- cells generated CD34+G- CSFR+ myeloid progenitors. In G-CSF-mobilized peripheral blood, CD34+ cells contained 10.8 ± 5.8% of G-CSFR+ cells, most of which were also myeloid progenitors, although CD34+G-CSFR- cells contained a substantial number of myeloid progenitors. These results indicated that the expression of G-CSFR on CD34+ cells is restricted to myeloid progenitors, suggesting that the specific activity of G-CSF on myelopoiesis depends on the exclusive expression of its receptor on myeloid progenitors, and that the mobilization of various haematopoietic progenitors is not a direct effect of G-CSF in humans.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalBritish Journal of Haematology
Volume109
Issue number1
StatePublished - 2000
Externally publishedYes

Fingerprint

Granulocyte Colony-Stimulating Factor Receptors
Gastrin-Secreting Cells
Granulocyte Colony-Stimulating Factor
Bone Marrow Cells
Myelopoiesis
Methylcellulose
Megakaryocytes
Cell Lineage
Cell Differentiation
Blood Cells
Bone Marrow
Cell Proliferation
Cytokines

Keywords

  • Bone marrow
  • CD34
  • G-CSF receptor
  • G-CSF-mobilized peripheral blood
  • Myeloid progenitor

ASJC Scopus subject areas

  • Hematology

Cite this

Ebihara, Y., Xu, M. J., Manabe, A., Kikuchi, A., Tanaka, R., Kato, S., ... Tsuji, K. (2000). Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34+ cells. British Journal of Haematology, 109(1), 153-161.

Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34+ cells. / Ebihara, Yasuhiro; Xu, Ming Jiang; Manabe, Atsushi; Kikuchi, Akira; Tanaka, Ryuhei; Kato, Shunichi; Nakahata, Tatsutoshi; Tsuji, Kohichiro.

In: British Journal of Haematology, Vol. 109, No. 1, 2000, p. 153-161.

Research output: Contribution to journalArticle

Ebihara, Y, Xu, MJ, Manabe, A, Kikuchi, A, Tanaka, R, Kato, S, Nakahata, T & Tsuji, K 2000, 'Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34+ cells', British Journal of Haematology, vol. 109, no. 1, pp. 153-161.
Ebihara Y, Xu MJ, Manabe A, Kikuchi A, Tanaka R, Kato S et al. Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34+ cells. British Journal of Haematology. 2000;109(1):153-161.
Ebihara, Yasuhiro ; Xu, Ming Jiang ; Manabe, Atsushi ; Kikuchi, Akira ; Tanaka, Ryuhei ; Kato, Shunichi ; Nakahata, Tatsutoshi ; Tsuji, Kohichiro. / Exclusive expression of G-CSF receptor on myeloid progenitors in bone marrow CD34+ cells. In: British Journal of Haematology. 2000 ; Vol. 109, No. 1. pp. 153-161.
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AU - Tanaka, Ryuhei

AU - Kato, Shunichi

AU - Nakahata, Tatsutoshi

AU - Tsuji, Kohichiro

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