Exercise acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells

Jalees Rehman, Jingling Li, Lakshmi Parvathaneni, Gudjon Karlsson, Vipul R. Panchal, Constance J. Temm, Jo Mahenthiran, Keith L. March

Research output: Contribution to journalArticle

241 Citations (Scopus)

Abstract

Objectives We investigated whether a single episode of exercise could acutely increase the numbers of endothelial progenitor cells (EPCs) and cultured/circulating angiogenic cells (CACs) in human subjects. Background Endothelial progenitor cells and CACs can be isolated from peripheral blood and have been shown to participate in vascular repair and angiogenesis. We hypothesized that exercise may acutely increase either circulating EPCs or CACs. Methods Volunteer subjects (n = 22) underwent exhaustive dynamic exercise. Blood was drawn before and after exercise, and circulating EPC numbers as well as plasma levels of angiogenic growth factors were assessed. The CACs were obtained by culturing mononuclear cells and the secretion of multiple angiogenic growth factors by CACs was determined. Results Circulating EPCs (AC133+/VE-Cadherin+ cells) increased nearly four-fold in peripheral blood from 66 ± 27 cells/ml to 236 ± 34 cells/ml (p < 0.05). The number of isolated CACs increased 2.5-fold from 8,754 ± 2,048 cells/ml of peripheral blood to 20,759 ± 4,676 cells/ml (p < 0.005). Cultured angiogenic cells isolated before and after exercise showed similar secretion patterns of angiogenic growth factors. Conclusions Our study demonstrates that exercise can acutely increase EPCs and CACs. Given the ability of these cell populations to promote angiogenesis and vascular regeneration, the exercise-induced cell mobilization may serve as a physiologic repair or compensation mechanism.

Original languageEnglish
Pages (from-to)2314-2318
Number of pages5
JournalJournal of the American College of Cardiology
Volume43
Issue number12
DOIs
StatePublished - Jun 16 2004

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Macrophages
Exercise
Angiogenesis Inducing Agents
Intercellular Signaling Peptides and Proteins
Endothelial Progenitor Cells
Blood Vessels
Regeneration
Volunteers
Cultured Cells
Cell Count

Keywords

  • CAC
  • cultured/circulating angiogenic cell
  • endothelial progenitor cell
  • EPC
  • G-CSF
  • GM-CSF
  • granulocyte colony-stimulating factor
  • granulocyte macrophage-colony stimulating factor
  • hepatocyte growth factor
  • HGF
  • LDL
  • low-density lipoprotein
  • MNC

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Exercise acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells. / Rehman, Jalees; Li, Jingling; Parvathaneni, Lakshmi; Karlsson, Gudjon; Panchal, Vipul R.; Temm, Constance J.; Mahenthiran, Jo; March, Keith L.

In: Journal of the American College of Cardiology, Vol. 43, No. 12, 16.06.2004, p. 2314-2318.

Research output: Contribution to journalArticle

Rehman, J, Li, J, Parvathaneni, L, Karlsson, G, Panchal, VR, Temm, CJ, Mahenthiran, J & March, KL 2004, 'Exercise acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells', Journal of the American College of Cardiology, vol. 43, no. 12, pp. 2314-2318. https://doi.org/10.1016/j.jacc.2004.02.049
Rehman, Jalees ; Li, Jingling ; Parvathaneni, Lakshmi ; Karlsson, Gudjon ; Panchal, Vipul R. ; Temm, Constance J. ; Mahenthiran, Jo ; March, Keith L. / Exercise acutely increases circulating endothelial progenitor cells and monocyte-/macrophage-derived angiogenic cells. In: Journal of the American College of Cardiology. 2004 ; Vol. 43, No. 12. pp. 2314-2318.
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AU - Parvathaneni, Lakshmi

AU - Karlsson, Gudjon

AU - Panchal, Vipul R.

AU - Temm, Constance J.

AU - Mahenthiran, Jo

AU - March, Keith L.

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N2 - Objectives We investigated whether a single episode of exercise could acutely increase the numbers of endothelial progenitor cells (EPCs) and cultured/circulating angiogenic cells (CACs) in human subjects. Background Endothelial progenitor cells and CACs can be isolated from peripheral blood and have been shown to participate in vascular repair and angiogenesis. We hypothesized that exercise may acutely increase either circulating EPCs or CACs. Methods Volunteer subjects (n = 22) underwent exhaustive dynamic exercise. Blood was drawn before and after exercise, and circulating EPC numbers as well as plasma levels of angiogenic growth factors were assessed. The CACs were obtained by culturing mononuclear cells and the secretion of multiple angiogenic growth factors by CACs was determined. Results Circulating EPCs (AC133+/VE-Cadherin+ cells) increased nearly four-fold in peripheral blood from 66 ± 27 cells/ml to 236 ± 34 cells/ml (p < 0.05). The number of isolated CACs increased 2.5-fold from 8,754 ± 2,048 cells/ml of peripheral blood to 20,759 ± 4,676 cells/ml (p < 0.005). Cultured angiogenic cells isolated before and after exercise showed similar secretion patterns of angiogenic growth factors. Conclusions Our study demonstrates that exercise can acutely increase EPCs and CACs. Given the ability of these cell populations to promote angiogenesis and vascular regeneration, the exercise-induced cell mobilization may serve as a physiologic repair or compensation mechanism.

AB - Objectives We investigated whether a single episode of exercise could acutely increase the numbers of endothelial progenitor cells (EPCs) and cultured/circulating angiogenic cells (CACs) in human subjects. Background Endothelial progenitor cells and CACs can be isolated from peripheral blood and have been shown to participate in vascular repair and angiogenesis. We hypothesized that exercise may acutely increase either circulating EPCs or CACs. Methods Volunteer subjects (n = 22) underwent exhaustive dynamic exercise. Blood was drawn before and after exercise, and circulating EPC numbers as well as plasma levels of angiogenic growth factors were assessed. The CACs were obtained by culturing mononuclear cells and the secretion of multiple angiogenic growth factors by CACs was determined. Results Circulating EPCs (AC133+/VE-Cadherin+ cells) increased nearly four-fold in peripheral blood from 66 ± 27 cells/ml to 236 ± 34 cells/ml (p < 0.05). The number of isolated CACs increased 2.5-fold from 8,754 ± 2,048 cells/ml of peripheral blood to 20,759 ± 4,676 cells/ml (p < 0.005). Cultured angiogenic cells isolated before and after exercise showed similar secretion patterns of angiogenic growth factors. Conclusions Our study demonstrates that exercise can acutely increase EPCs and CACs. Given the ability of these cell populations to promote angiogenesis and vascular regeneration, the exercise-induced cell mobilization may serve as a physiologic repair or compensation mechanism.

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