Exercise training alters Ca release from coronary smooth muscle sarcoplasmic reticulum

L. Stehno-Bittel, M. H. Laughlin, Michael Sturek

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We examined intracellular free calcium (Ca(i)) buffering by the sarcoplasmic reticulum (SR) of porcine coronary artery smooth muscle cellse (SMC) and the effects of chronic exercise training (ET) on this phenomenon. Ca(i) was measured with fura-2. Depolarization of single SMC via high extracellular K induced the entry of Ca, a fraction of which was sequestered (buffered) by the SR. Endurance ET of Yucatan minipigs for 16-20 wk increased exercise tolerance, heart-to-body weight ratios, and skeletal muscle oxidative capacity. Caffeine-induced Ca release from the SR after a short (2 min) recovery time from depolarization to caffeine exposure was equal in SMC from both ET and sedentary pigs. With a longer (11 min) recovery period after depolarization, caffeine-induced Ca release from the SR of sedentary pigs was equal to that after the short recovery time. However, after the long recovery, caffeine-induced Ca release in SMC of ET pigs was 60% less (P

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume259
Issue number2 28-2
StatePublished - 1990
Externally publishedYes

Fingerprint

Sarcoplasmic Reticulum
Caffeine
Smooth Muscle
Swine
Exercise
Calcium
Miniature Swine
Exercise Tolerance
Fura-2
Coronary Vessels
Skeletal Muscle
Body Weight

Keywords

  • Ca influx
  • caffeine
  • pigs
  • voltage-gated Ca channel

ASJC Scopus subject areas

  • Physiology

Cite this

Exercise training alters Ca release from coronary smooth muscle sarcoplasmic reticulum. / Stehno-Bittel, L.; Laughlin, M. H.; Sturek, Michael.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 259, No. 2 28-2, 1990.

Research output: Contribution to journalArticle

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