Exercise training restores adenosine-induced relaxation in coronary arteries distal to chronic occlusion

Cristine L. Heaps, Michael Sturek, Julie A. Rapps, M. Harold Laughlin, Janet L. Parker

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We previously reported that canine collateral-dependent coronary arteries exhibit impaired relaxation to adenosine but not sodium nitroprusside. In contrast, exercise training enhances adenosine sensitivity of normal porcine coronary arteries. These results stimulated the hypothesis that chronic coronary occlusion and exercise training produce differential effects on cAMP-versus cGMP-mediated relaxation. To test this hypothesis, Ameroid occluders were surgically placed around the proximal left circumflex coronary artery (LCx) of female Yucatan miniature swine 8 wk before initiating sedentary or exercise training (treadmill run, 16 wk) protocols. Relaxation to the cAMP-dependent vasodilators adenosine (10-7 to 10-3 M) and isoproterenol (3 x 10-8 to 3 x 10-5 M) were impaired in collateral- dependent LCx versus nonoccluded left anterior descending (LAD) arterial rings isolated from sedentary but not exercise-trained pigs. Furthermore, adenosine-mediated reductions in simultaneous tension and myoplasmic free Ca2+ were impaired in LCx versus LAD arteries isolated from sedentary but not exercise-trained pigs. In contrast, relaxation in response to the cAMP- dependent vasodilator forskolin (10-9 to 10-5 M) and the cGMP-dependent vasodilator sodium nitroprusside (10-9 to 10-4 M) was not different in LCx versus LAD arteries of sedentary or exercise-trained animals. These data suggest that chronic occlusion impairs receptor-dependent, cAMP-mediated relaxation; receptor-independent cAMP- and cGMP-mediated relaxation were unimpaired. Importantly, exercise training restores cAMP-mediated relaxation of collateral-dependent coronary arteries.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume278
Issue number6 47-6
StatePublished - Jun 2000
Externally publishedYes

Fingerprint

Adenosine
Coronary Vessels
Vasodilator Agents
Cyclic AMP Receptors
Exercise
Swine
Nitroprusside
Arteries
Miniature Swine
Coronary Occlusion
Colforsin
Isoproterenol
Canidae

Keywords

  • Adenosine 3',5'-cyclic monophosphate
  • Collateral dependent
  • Guanosine 3', 5'-cyclic monophosphate
  • Myoplasmic free calcium
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Exercise training restores adenosine-induced relaxation in coronary arteries distal to chronic occlusion. / Heaps, Cristine L.; Sturek, Michael; Rapps, Julie A.; Laughlin, M. Harold; Parker, Janet L.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 278, No. 6 47-6, 06.2000.

Research output: Contribution to journalArticle

@article{470fe2041e89439ab689548cf4f566fe,
title = "Exercise training restores adenosine-induced relaxation in coronary arteries distal to chronic occlusion",
abstract = "We previously reported that canine collateral-dependent coronary arteries exhibit impaired relaxation to adenosine but not sodium nitroprusside. In contrast, exercise training enhances adenosine sensitivity of normal porcine coronary arteries. These results stimulated the hypothesis that chronic coronary occlusion and exercise training produce differential effects on cAMP-versus cGMP-mediated relaxation. To test this hypothesis, Ameroid occluders were surgically placed around the proximal left circumflex coronary artery (LCx) of female Yucatan miniature swine 8 wk before initiating sedentary or exercise training (treadmill run, 16 wk) protocols. Relaxation to the cAMP-dependent vasodilators adenosine (10-7 to 10-3 M) and isoproterenol (3 x 10-8 to 3 x 10-5 M) were impaired in collateral- dependent LCx versus nonoccluded left anterior descending (LAD) arterial rings isolated from sedentary but not exercise-trained pigs. Furthermore, adenosine-mediated reductions in simultaneous tension and myoplasmic free Ca2+ were impaired in LCx versus LAD arteries isolated from sedentary but not exercise-trained pigs. In contrast, relaxation in response to the cAMP- dependent vasodilator forskolin (10-9 to 10-5 M) and the cGMP-dependent vasodilator sodium nitroprusside (10-9 to 10-4 M) was not different in LCx versus LAD arteries of sedentary or exercise-trained animals. These data suggest that chronic occlusion impairs receptor-dependent, cAMP-mediated relaxation; receptor-independent cAMP- and cGMP-mediated relaxation were unimpaired. Importantly, exercise training restores cAMP-mediated relaxation of collateral-dependent coronary arteries.",
keywords = "Adenosine 3',5'-cyclic monophosphate, Collateral dependent, Guanosine 3', 5'-cyclic monophosphate, Myoplasmic free calcium, Vascular smooth muscle",
author = "Heaps, {Cristine L.} and Michael Sturek and Rapps, {Julie A.} and Laughlin, {M. Harold} and Parker, {Janet L.}",
year = "2000",
month = "6",
language = "English (US)",
volume = "278",
journal = "American Journal of Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "6 47-6",

}

TY - JOUR

T1 - Exercise training restores adenosine-induced relaxation in coronary arteries distal to chronic occlusion

AU - Heaps, Cristine L.

AU - Sturek, Michael

AU - Rapps, Julie A.

AU - Laughlin, M. Harold

AU - Parker, Janet L.

PY - 2000/6

Y1 - 2000/6

N2 - We previously reported that canine collateral-dependent coronary arteries exhibit impaired relaxation to adenosine but not sodium nitroprusside. In contrast, exercise training enhances adenosine sensitivity of normal porcine coronary arteries. These results stimulated the hypothesis that chronic coronary occlusion and exercise training produce differential effects on cAMP-versus cGMP-mediated relaxation. To test this hypothesis, Ameroid occluders were surgically placed around the proximal left circumflex coronary artery (LCx) of female Yucatan miniature swine 8 wk before initiating sedentary or exercise training (treadmill run, 16 wk) protocols. Relaxation to the cAMP-dependent vasodilators adenosine (10-7 to 10-3 M) and isoproterenol (3 x 10-8 to 3 x 10-5 M) were impaired in collateral- dependent LCx versus nonoccluded left anterior descending (LAD) arterial rings isolated from sedentary but not exercise-trained pigs. Furthermore, adenosine-mediated reductions in simultaneous tension and myoplasmic free Ca2+ were impaired in LCx versus LAD arteries isolated from sedentary but not exercise-trained pigs. In contrast, relaxation in response to the cAMP- dependent vasodilator forskolin (10-9 to 10-5 M) and the cGMP-dependent vasodilator sodium nitroprusside (10-9 to 10-4 M) was not different in LCx versus LAD arteries of sedentary or exercise-trained animals. These data suggest that chronic occlusion impairs receptor-dependent, cAMP-mediated relaxation; receptor-independent cAMP- and cGMP-mediated relaxation were unimpaired. Importantly, exercise training restores cAMP-mediated relaxation of collateral-dependent coronary arteries.

AB - We previously reported that canine collateral-dependent coronary arteries exhibit impaired relaxation to adenosine but not sodium nitroprusside. In contrast, exercise training enhances adenosine sensitivity of normal porcine coronary arteries. These results stimulated the hypothesis that chronic coronary occlusion and exercise training produce differential effects on cAMP-versus cGMP-mediated relaxation. To test this hypothesis, Ameroid occluders were surgically placed around the proximal left circumflex coronary artery (LCx) of female Yucatan miniature swine 8 wk before initiating sedentary or exercise training (treadmill run, 16 wk) protocols. Relaxation to the cAMP-dependent vasodilators adenosine (10-7 to 10-3 M) and isoproterenol (3 x 10-8 to 3 x 10-5 M) were impaired in collateral- dependent LCx versus nonoccluded left anterior descending (LAD) arterial rings isolated from sedentary but not exercise-trained pigs. Furthermore, adenosine-mediated reductions in simultaneous tension and myoplasmic free Ca2+ were impaired in LCx versus LAD arteries isolated from sedentary but not exercise-trained pigs. In contrast, relaxation in response to the cAMP- dependent vasodilator forskolin (10-9 to 10-5 M) and the cGMP-dependent vasodilator sodium nitroprusside (10-9 to 10-4 M) was not different in LCx versus LAD arteries of sedentary or exercise-trained animals. These data suggest that chronic occlusion impairs receptor-dependent, cAMP-mediated relaxation; receptor-independent cAMP- and cGMP-mediated relaxation were unimpaired. Importantly, exercise training restores cAMP-mediated relaxation of collateral-dependent coronary arteries.

KW - Adenosine 3',5'-cyclic monophosphate

KW - Collateral dependent

KW - Guanosine 3', 5'-cyclic monophosphate

KW - Myoplasmic free calcium

KW - Vascular smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=0033921995&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033921995&partnerID=8YFLogxK

M3 - Article

C2 - 10843897

AN - SCOPUS:0033921995

VL - 278

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1857

IS - 6 47-6

ER -