Exosome-associated hepatitis C virus in cell cultures and patient plasma

Ziqing Liu, Xiugen Zhang, Andy Yu, Johnny J. He

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-cell contact. Here we report that HCV is associated with exosomes. Using highly purified exosomes and transmission electron microscopic imaging, we demonstrated that HCV occurred in both exosome-free and exosome-associated forms. Exosome-associated HCV was infectious and resistant to neutralization by an anti-HCV neutralizing antibody. There were more exosome-associated HCV than exosome-free HCV detected in the plasma of HCV-infected patients. These results suggest exosome-associated HCV as an alternative form for HCV infection and transmission.

Original languageEnglish
Pages (from-to)218-222
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume455
Issue number3-4
DOIs
StatePublished - Dec 12 2014

Fingerprint

Exosomes
Satellite Viruses
Viruses
Cell culture
Hepacivirus
Cell Culture Techniques
Plasmas
Infectious Disease Transmission
Hepatitis C Antibodies
Virus Diseases
Neutralizing Antibodies
Electrons

Keywords

  • Exosome
  • HCV
  • Infection
  • Neutralizing antibody
  • Patient plasma
  • TEM imaging

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Exosome-associated hepatitis C virus in cell cultures and patient plasma. / Liu, Ziqing; Zhang, Xiugen; Yu, Andy; He, Johnny J.

In: Biochemical and Biophysical Research Communications, Vol. 455, No. 3-4, 12.12.2014, p. 218-222.

Research output: Contribution to journalArticle

Liu, Ziqing ; Zhang, Xiugen ; Yu, Andy ; He, Johnny J. / Exosome-associated hepatitis C virus in cell cultures and patient plasma. In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 455, No. 3-4. pp. 218-222.
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