Expansion of specialized epidermis induced by hormonal state and mechanical strain

Hsin Jung Wu, Teresa Easwaran, Carlos D. Offutt, Richard Levi Elgar, Dan Spandau, Sachiko Koyama, John Foley

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In mammals, some sites of specialized skin such as the palms, soles, and lips grow proportionally with the animal. However, other types of specialized skin such as the nipple and anal/genital region are dramatically altered with changes of reproductive status. The specific cell types that mediate the growth of these sites have not been identified. In the mouse, we observed a dramatic expansion of the specialized epidermis of the nipple, coupled to changes in connective tissue and hair shaft density, which we designate as areola formation. During this process thymidine analog uptake was elevated in the epidermis and hair follicles. Although there were no changes in connective tissue cell proliferation, we did observe an altered expression of extracellular matrix genes. In addition, the fibroblasts of the virgin nipple areola and region showed increased transcript and protein levels for estrogen, progesterone, relaxin, and oxytocin relative to those of ventral skin. To determine the role of pregnancy, lactation hormonal milieu, and localized mechanical strain on areola formation, we created models that separated these stimuli and evaluated changes in gross structure, proliferation and protein expression. While modest increases of epidermal proliferation and remodeling of connective tissue occurred as a result of individual stimuli, areola formation required exposure to pregnancy hormones, as well as mechanical strain.

Original languageEnglish
Pages (from-to)73-86
Number of pages14
JournalMechanisms of Development
Volume136
DOIs
StatePublished - May 1 2015

Fingerprint

Nipples
Epidermis
Connective Tissue
Skin
Relaxin
Connective Tissue Cells
Pregnancy
Hair Follicle
Oxytocin
Lip
Lactation
Hair
Thymidine
Extracellular Matrix
Progesterone
Mammals
Estrogens
Proteins
Fibroblasts
Cell Proliferation

Keywords

  • Areola
  • Hormones
  • Lactation
  • Mechanical strain
  • Nipple
  • Pregnancy

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

Cite this

Expansion of specialized epidermis induced by hormonal state and mechanical strain. / Wu, Hsin Jung; Easwaran, Teresa; Offutt, Carlos D.; Elgar, Richard Levi; Spandau, Dan; Koyama, Sachiko; Foley, John.

In: Mechanisms of Development, Vol. 136, 01.05.2015, p. 73-86.

Research output: Contribution to journalArticle

Wu, Hsin Jung ; Easwaran, Teresa ; Offutt, Carlos D. ; Elgar, Richard Levi ; Spandau, Dan ; Koyama, Sachiko ; Foley, John. / Expansion of specialized epidermis induced by hormonal state and mechanical strain. In: Mechanisms of Development. 2015 ; Vol. 136. pp. 73-86.
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