Experimental assessment of small intestinal submucosa as a bladder wall substitute

Bradley P. Kropp, Barry L. Eppley, C. D. Prevel, M. K. Rippy, R. C. Harruff, S. F. Badylak, M. C. Adams, R. C. Rink, M. A. Keating

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Abstract

Objectives. This study determined the feasibility of promoting urinary bladder regeneration with porcine-derived small intestinal submucosa (SIS). Methods. Twenty-two Sprague-Dawley rats underwent partial cystectomy with immediate bladder augmentation with SIS. Bladders were harvested for histologic evaluation at 2, 4, 8, 12, 24, and 48 weeks. Results. Histologically at 2 weeks, there was infiltration of the graft material with viable host cells consisting of fibroblasts, macrophages, and blood vessels covered by complete mucosal urothelium comprised of transitional cells. During the next 10 weeks, collagen formation and maturation were noted, and by the end of 12 weeks, the SIS graft was comprised of a mature collagen matrix admixed with thinly scattered disorganized smooth muscle bundles and covered by normal urothelium. At 48 weeks, all three layers of the normal bladder (urothelium, smooth muscle, and serosa) were present and were grossly and microscopically indistinguishable from the normal rat urinary bladder. Conclusions. This study further supports the concept of bladder regeneration and suggests that SIS may be a viable material for bladder augmentations.

Original languageEnglish
Pages (from-to)396-400
Number of pages5
JournalUrology
Volume46
Issue number3
DOIs
StatePublished - 1995

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Urinary Bladder
Urothelium
Smooth Muscle
Regeneration
Collagen
Transplants
Serous Membrane
Cystectomy
Feasibility Studies
Blood Vessels
Sprague Dawley Rats
Swine
Fibroblasts
Macrophages

ASJC Scopus subject areas

  • Urology

Cite this

Kropp, B. P., Eppley, B. L., Prevel, C. D., Rippy, M. K., Harruff, R. C., Badylak, S. F., ... Keating, M. A. (1995). Experimental assessment of small intestinal submucosa as a bladder wall substitute. Urology, 46(3), 396-400. https://doi.org/10.1016/S0090-4295(99)80227-1

Experimental assessment of small intestinal submucosa as a bladder wall substitute. / Kropp, Bradley P.; Eppley, Barry L.; Prevel, C. D.; Rippy, M. K.; Harruff, R. C.; Badylak, S. F.; Adams, M. C.; Rink, R. C.; Keating, M. A.

In: Urology, Vol. 46, No. 3, 1995, p. 396-400.

Research output: Contribution to journalArticle

Kropp, BP, Eppley, BL, Prevel, CD, Rippy, MK, Harruff, RC, Badylak, SF, Adams, MC, Rink, RC & Keating, MA 1995, 'Experimental assessment of small intestinal submucosa as a bladder wall substitute', Urology, vol. 46, no. 3, pp. 396-400. https://doi.org/10.1016/S0090-4295(99)80227-1
Kropp BP, Eppley BL, Prevel CD, Rippy MK, Harruff RC, Badylak SF et al. Experimental assessment of small intestinal submucosa as a bladder wall substitute. Urology. 1995;46(3):396-400. https://doi.org/10.1016/S0090-4295(99)80227-1
Kropp, Bradley P. ; Eppley, Barry L. ; Prevel, C. D. ; Rippy, M. K. ; Harruff, R. C. ; Badylak, S. F. ; Adams, M. C. ; Rink, R. C. ; Keating, M. A. / Experimental assessment of small intestinal submucosa as a bladder wall substitute. In: Urology. 1995 ; Vol. 46, No. 3. pp. 396-400.
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AU - Harruff, R. C.

AU - Badylak, S. F.

AU - Adams, M. C.

AU - Rink, R. C.

AU - Keating, M. A.

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N2 - Objectives. This study determined the feasibility of promoting urinary bladder regeneration with porcine-derived small intestinal submucosa (SIS). Methods. Twenty-two Sprague-Dawley rats underwent partial cystectomy with immediate bladder augmentation with SIS. Bladders were harvested for histologic evaluation at 2, 4, 8, 12, 24, and 48 weeks. Results. Histologically at 2 weeks, there was infiltration of the graft material with viable host cells consisting of fibroblasts, macrophages, and blood vessels covered by complete mucosal urothelium comprised of transitional cells. During the next 10 weeks, collagen formation and maturation were noted, and by the end of 12 weeks, the SIS graft was comprised of a mature collagen matrix admixed with thinly scattered disorganized smooth muscle bundles and covered by normal urothelium. At 48 weeks, all three layers of the normal bladder (urothelium, smooth muscle, and serosa) were present and were grossly and microscopically indistinguishable from the normal rat urinary bladder. Conclusions. This study further supports the concept of bladder regeneration and suggests that SIS may be a viable material for bladder augmentations.

AB - Objectives. This study determined the feasibility of promoting urinary bladder regeneration with porcine-derived small intestinal submucosa (SIS). Methods. Twenty-two Sprague-Dawley rats underwent partial cystectomy with immediate bladder augmentation with SIS. Bladders were harvested for histologic evaluation at 2, 4, 8, 12, 24, and 48 weeks. Results. Histologically at 2 weeks, there was infiltration of the graft material with viable host cells consisting of fibroblasts, macrophages, and blood vessels covered by complete mucosal urothelium comprised of transitional cells. During the next 10 weeks, collagen formation and maturation were noted, and by the end of 12 weeks, the SIS graft was comprised of a mature collagen matrix admixed with thinly scattered disorganized smooth muscle bundles and covered by normal urothelium. At 48 weeks, all three layers of the normal bladder (urothelium, smooth muscle, and serosa) were present and were grossly and microscopically indistinguishable from the normal rat urinary bladder. Conclusions. This study further supports the concept of bladder regeneration and suggests that SIS may be a viable material for bladder augmentations.

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