Experimental human infection with Haemophilus ducreyi

Stanley Spinola, Linda M. Wild, Michael A. Apicella, Anthony A. Gaspari, Anthony A. Campagnari

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Four subjects were experimentally infected with Haemophilus ducreyi. Lesions developed only at sites where live bacteria were inoculated on abraded skin. No subject developed fever, lymphadenopathy, or disseminated infection during a 3-day observation period. Two subjects who were rechallenged 2 months after initial infection also developed lesions. The amount of H. ducreyi recovered from 10 of 12 biopsies that were semiquantitatively cultured varied widely. Similar histologic features were present in initial and second infections. The epidermis contained pustules; the dermis contained an infiltrate of T cells and macrophages and reactive endothelial cells. Keratinocytes and T cells expressed HLA-DR, consistent with a delayed-type hypersensitivity response. The subjects did not mount humoral responses to bacterial proteins and to lipooligosaccharides after primary and secondary challenges. Thus, human experimental infection with H. ducreyi is well tolerated and safe. Recruitment of T cells and macrophages into chancroid lesions may partially explain the association between chancroid and human immunodeficiency virus transmission.

Original languageEnglish
Pages (from-to)1146-1150
Number of pages5
JournalJournal of Infectious Diseases
Volume169
Issue number5
StatePublished - May 1994

Fingerprint

Haemophilus ducreyi
Chancroid
Infection
T-Lymphocytes
Macrophages
Bacterial Proteins
Delayed Hypersensitivity
HLA-DR Antigens
Dermis
Keratinocytes
Epidermis
Fever
Endothelial Cells
Observation
HIV
Bacteria
Biopsy
Skin

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Spinola, S., Wild, L. M., Apicella, M. A., Gaspari, A. A., & Campagnari, A. A. (1994). Experimental human infection with Haemophilus ducreyi. Journal of Infectious Diseases, 169(5), 1146-1150.

Experimental human infection with Haemophilus ducreyi. / Spinola, Stanley; Wild, Linda M.; Apicella, Michael A.; Gaspari, Anthony A.; Campagnari, Anthony A.

In: Journal of Infectious Diseases, Vol. 169, No. 5, 05.1994, p. 1146-1150.

Research output: Contribution to journalArticle

Spinola, S, Wild, LM, Apicella, MA, Gaspari, AA & Campagnari, AA 1994, 'Experimental human infection with Haemophilus ducreyi', Journal of Infectious Diseases, vol. 169, no. 5, pp. 1146-1150.
Spinola S, Wild LM, Apicella MA, Gaspari AA, Campagnari AA. Experimental human infection with Haemophilus ducreyi. Journal of Infectious Diseases. 1994 May;169(5):1146-1150.
Spinola, Stanley ; Wild, Linda M. ; Apicella, Michael A. ; Gaspari, Anthony A. ; Campagnari, Anthony A. / Experimental human infection with Haemophilus ducreyi. In: Journal of Infectious Diseases. 1994 ; Vol. 169, No. 5. pp. 1146-1150.
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