Experimental infection of human volunteers with Haemophilus ducreyi: Fifteen years of clinical data and experience

Diane M. Janowicz, Susan Ofner, Barry P. Katz, Stanley M. Spinola

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Haemophilus ducreyi causes chancroid, which facilitates transmission of human immunodeficiency virus type 1. To better understand the biology of H. ducreyi, we developed a human inoculation model. In the present article, we describe clinical outcomes for 267 volunteers who were infected with H. ducreyi. There was a relationship between papule formation and estimated delivered dose. The outcome (either pustule formation or resolution) of infected sites for a given subject was not independent; the most important determinants of pustule formation were sex and host effects. When 41 subjects were infected a second time, their outcomes segregated toward their initial outcome, confirming the host effect. Subjects with pustules developed local symptoms that required withdrawal from the study after a mean of 8.6 days. There were 191 volunteers who had tissue biopsy performed, 173 of whom were available for follow-up analysis; 28 (16.2%) of these developed hypertrophic scars, but the model was otherwise safe. Mutant-parent trials confirmed key features in H. ducreyi pathogenesis, and the model has provided an opportunity to study differential human susceptibility to a bacterial infection.

Original languageEnglish (US)
Pages (from-to)1671-1679
Number of pages9
JournalJournal of Infectious Diseases
Volume199
Issue number11
DOIs
StatePublished - Jun 1 2009

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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